Evidence map›Paper›PMID 38957789›Full record

ArticleiScience2024

An HIV-1 CRISPR-Cas9 membrane trafficking screen reveals a role for PICALM intersecting endolysosomes and immunity.

Paola Guizar, Ana Luiza Abdalla, Anne Monette, Kristin Davis, Ramon Edwin Caballero, Meijuan Niu, Xinyun Liu, Oluwaseun Ajibola, Thomas T Murooka, Chen Liang and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Paola GuizarLady Davis Institute at the Jewish General Hospital, Montréal, QC H3T 1E2, Canada.
Ana Luiza AbdallaLady Davis Institute at the Jewish General Hospital, Montréal, QC H3T 1E2, Canada.
Anne MonetteLady Davis Institute at the Jewish General Hospital, Montréal, QC H3T 1E2, Canada.
Kristin DavisLady Davis Institute at the Jewish General Hospital, Montréal, QC H3T 1E2, Canada.
Ramon Edwin CaballeroDepartment of Microbiology and Immunology, McGill University, Montréal, QC H3A 2B4, Canada.
Meijuan NiuLady Davis Institute at the Jewish General Hospital, Montréal, QC H3T 1E2, Canada.
Xinyun LiuRady Faculty of Health Science, Department of Immunology, University of Manitoba, Winnipeg, MB R3E 0T5, Canada.
Oluwaseun AjibolaRady Faculty of Health Science, Department of Immunology, University of Manitoba, Winnipeg, MB R3E 0T5, Canada.
Thomas T MurookaRady Faculty of Health Science, Department of Immunology, University of Manitoba, Winnipeg, MB R3E 0T5, Canada.
Chen LiangLady Davis Institute at the Jewish General Hospital, Montréal, QC H3T 1E2, Canada.
Andrew J MoulandLady Davis Institute at the Jewish General Hospital, Montréal, QC H3T 1E2, Canada.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

HIV-1 hijacks host proteins involved in membrane trafficking, endocytosis, and autophagy that are critical for virus replication. Molecular details are lacking but are essential to inform on the development of alternative antiviral strategies. Despite their potential as clinical targets, only a few membrane trafficking proteins have been functionally characterized in HIV-1 replication. To further elucidate roles in HIV-1 replication, we performed a CRISPR-Cas9 screen on 140 membrane trafficking proteins. We identified phosphatidylinositol-binding clathrin assembly protein (PICALM) that influences not only infection dynamics but also CD4

Indexed as

Cell biologyHuman geneticsVirology

Identifiers

PMID38957789
PMCPMC11217618

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.