Evidence map›Paper›PMID 38957035›Full record

ArticleJournal of cellular and molecular medicine2024

LPS exacerbates TRPV4-mediated itch through the intracellular TLR4-PI3K signalling.

Yanping Hao, Liyan Wu, Yuhui Wang, Dongmei Shan, Yifei Liu, Jing Feng, Yi Chang, Ting Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cellular and molecular medicine, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yanping HaoShanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.
Liyan WuShanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.
Yuhui WangDepartment of Anesthesiology, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Dongmei ShanShanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.
Yifei LiuShanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.
Jing FengShanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.
Yi ChangYangpu Hospital, School of Medicine, Tongji University, Shanghai, China.
Ting WangShanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.ORCID 0000-0003-4717-9530

Funding

Lingang Laboratory LG-QS-202203-07National Natural Science Foundation of China 82171214,32241003Natural Science Foundation of Shanghai Municipality 23ZR1474500Research Project of Yunnan Key Laboratory of Gastrodia and Fungi Symbiotic Biology TMKF2024B01
6 · The paper itself

Abstract

Pruritus is often accompanied with bacterial infections, but the underlying mechanism is not fully understood. Although previous studies revealed that lipopolysaccharides (LPS) could directly activate TRPV4 channel and TRPV4 is involved in the generation of both acute itch and chronic itch, whether and how LPS affects TRPV4-mediated itch sensation remains unclear. Here, we showed that LPS-mediated TRPV4 sensitization exacerbated GSK101-induced scratching behaviour in mice. Moreover, this effect was compromised in TLR4-knockout mice, suggesting LPS acted through a TLR4-dependent mechanism. Mechanistically, LPS enhanced GSK101-evoked calcium influx in mouse ear skin cells and HEK293T cells transfected with TRPV4. Further, LPS sensitized TRPV4 channel through the intracellular TLR4-PI3K-AKT signalling. In summary, our study found a modulatory role of LPS in TRPV4 function and highlighted the TLR4-TRPV4 interaction in itch signal amplification.

Indexed as

LipopolysaccharidesPhosphatidylinositol 3-KinasesPruritusSignal TransductionToll-Like Receptor 4TRPV Cation ChannelsAnimalsCalciumHEK293 CellsHumansMaleMiceMice, Inbred C57BLMice, KnockoutProto-Oncogene Proteins c-aktCalciumLipopolysaccharidesPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktTlr4 protein, mouseToll-Like Receptor 4Trpv4 protein, mouseTRPV Cation Channelsitch sensitizationLPSPI3KTLR4TRPV4

Identifiers

PMID38957035
PMCPMC11220342

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.