Evidence map›Paper›PMID 38956701›Full record

ArticleJournal of animal science and biotechnology2024

Progesterone regulates tissue non-specific alkaline phosphatase (TNSALP) expression and activity in ovine utero-placental tissues.

Claire Stenhouse, Katherine M Halloran, Emily C Hoskins, Robyn M Moses, Guoyao Wu, Heewon Seo, Gregory A Johnson, Larry J Suva, Dana Gaddy, Fuller W Bazer

Abstract read
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In one paragraph

Article in Journal of animal science and biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Claire StenhouseDepartment of Animal Science, Pennsylvania State University, University Park, PA, 16802, U.S.A.. cms9086@psu.edu.
Katherine M HalloranDepartment of Animal Science, Texas A&M University, College Station, Texas, 77843, U.S.A.
Emily C HoskinsDepartment of Animal Science, Texas A&M University, College Station, Texas, 77843, U.S.A.
Robyn M MosesDepartment of Animal Science, Texas A&M University, College Station, Texas, 77843, U.S.A.
Guoyao WuDepartment of Animal Science, Texas A&M University, College Station, Texas, 77843, U.S.A.
Heewon SeoDepartment of Veterinary Integrative Biosciences, Texas A&M University, College Station, Texas, 77843, U.S.A.
Gregory A JohnsonDepartment of Veterinary Integrative Biosciences, Texas A&M University, College Station, Texas, 77843, U.S.A.
Larry J SuvaDepartment of Veterinary Physiology and Pharmacology, Texas A&M University, College Station, Texas, 77843, U.S.A.
Dana GaddyDepartment of Veterinary Integrative Biosciences, Texas A&M University, College Station, Texas, 77843, U.S.A.
Fuller W BazerDepartment of Animal Science, Texas A&M University, College Station, Texas, 77843, U.S.A.

Funding

National Institute of Food and Agriculture 2016-67015-24958
6 · The paper itself

Abstract

backgroundTissue non-specific alkaline phosphatase (TNSALP; encoded by the ALPL gene) has a critical role in the postnatal regulation of phosphate homeostasis, yet how TNSALP activity and expression are regulated during pregnancy remain largely unknown. This study tested the hypothesis that progesterone (P4) and/or interferon tau (IFNT) regulate TNSALP activity during pregnancy in sheep.

methodsIn Exp. 1, ewes were bred and received daily intramuscular injections of either corn oil vehicle (CO) or 25 mg progesterone in CO (P4) for the first 8 days of pregnancy and were hysterectomized on either Day 9, 12, or 125 of gestation. In Exp. 2, ewes were fitted with intrauterine catheters on Day 7 of the estrous cycle and received daily intramuscular injections of 50 mg P4 in CO and/or 75 mg progesterone receptor antagonist (RU486) in CO from Days 8 to 15, and twice daily intrauterine injections of either control proteins (CX) or IFNT (25 µg/uterine horn/d) from Days 11 to 15 (treatment groups: P4 + CX; P4 + IFNT; RU486 + P4 + CX; and RU486 + P4 + IFNT) and were hysterectomized on Day 16.

resultsIn Exp. 1, endometria from ewes administered P4 had greater expression of ALPL mRNA than ewes administered CO on Day 12. TNSALP activity appeared greater in the epithelia, stratum compactum stroma, and endothelium of the blood vessels in the endometrium and myometrium from ewes administered P4 than ewes administered CO on Day 12. On Day 125, TNSALP activity localized to uterine epithelial and endothelial cells, independent of P4 treatment. TNSALP activity in placentomes appeared greater in P4 treated ewes and was detected in endothelial cells and caruncular tissue in P4 treated but not CO treated ewes. In Exp. 2, endometrial homogenates from ewes administered RU486 + P4 + CX had lower TNSALP activity those for P4 + CX and P4 + IFNT ewes. Immunoreactive TNSALP protein appeared greater in the mid- and deep-glandular epithelia in RU486 + P4 + CX treated ewes as compared to the other treatment groups. Enzymatic activity appeared greater on the apical surface of the deep glandular epithelia in endometria from ewes treated with RU486 + P4 + CX compared to the other treatment groups.

conclusionsThese results suggest that P4, but not IFNT, regulates the expression and activity of TNSALP in utero-placental tissues and has the potential to contribute to the regulation of phosphate availability that is critical for conceptus development during pregnancy.

Indexed as

Alkaline phosphatasePhosphatePlacentaSheepUterus

Identifiers

PMID38956701
PMCPMC11220967

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.