Evidence map›Paper›PMID 38956381›Full record

ReviewNature protocols2024

Observing isolated synaptic vesicle association and fusion ex vivo.

Jeremy Leitz, Chuchu Wang, Luis Esquivies, John J Peters, Nisha Gopal, Richard A Pfuetzner, Austin L Wang, Axel T Brunger

Erratum issuedAbstract readReview
PubMed Publisher
In one paragraph

Review in Nature protocols, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. Cryo-EM single-particle analysis expanding towards increasingly native samples.Acta crystallographica. Section D, Structural biology · 2025
    Review
  5. Article
  6. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Jeremy LeitzDepartment of Molecular and Cellular Physiology, Stanford University, Stanford, CA, USA.
Chuchu WangDepartment of Molecular and Cellular Physiology, Stanford University, Stanford, CA, USA.ORCID 0000-0003-2015-7331
Luis EsquiviesDepartment of Molecular and Cellular Physiology, Stanford University, Stanford, CA, USA.
John J PetersDepartment of Molecular and Cellular Physiology, Stanford University, Stanford, CA, USA.
Nisha GopalDepartment of Molecular and Cellular Physiology, Stanford University, Stanford, CA, USA.ORCID 0000-0003-1980-2292
Richard A PfuetznerDepartment of Molecular and Cellular Physiology, Stanford University, Stanford, CA, USA.
Austin L WangDepartment of Molecular and Cellular Physiology, Stanford University, Stanford, CA, USA.
Axel T BrungerDepartment of Molecular and Cellular Physiology, Stanford University, Stanford, CA, USA. brunger@stanford.edu.ORCID 0000-0001-5121-2036

Funding

STRUCTURE OF PROTEINS INVOLVED IN SYNAPTIC EXOCYTOSISR01MH063105 · NIMH · STANFORD UNIVERSITY · PI BRUNGER, AXEL T · 2000 to 2025
$4.7M
Foundation for the National Institutes of Health (Foundation for the National Institutes of Health, Inc.) RO1MH63105Howard Hughes Medical Institute (HHMI) n/aNIMH NIH HHS R01 MH063105
6 · The paper itself

Abstract

Here, we present a protocol for isolating functionally intact glutamatergic synaptic vesicles from whole-mouse brain tissue and using them in a single-vesicle assay to examine their association and fusion with plasma membrane mimic vesicles. This is a Protocol Extension, building on our previous protocol, which used a purely synthetic system comprised of reconstituted proteins in liposomes. We also describe the generation of a peptide based on the vesicular glutamate transporter, which is essential in the isolation process of glutamatergic synaptic vesicles. This method uses easily accessible reagents to generate fusion-competent glutamatergic synaptic vesicles through immunoisolation. The generation of the vGlut peptide can be accomplished in 6 d, while the isolation of the synaptic vesicles by using the peptide can be accomplished in 2 d, with an additional day to fluorescently label the synaptic vesicles for use in a single-vesicle hybrid fusion assay. The single-vesicle fusion assay can be accomplished in 1 d and can unambiguously delineate synaptic vesicle association, dissociation, Ca

Indexed as

Membrane FusionSynaptic VesiclesAnimalsBrainMice

Identifiers

PMID38956381

What OpenQuestion holds

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Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.