Evidence map›Paper›PMID 38956138›Full record

ArticleScientific reports2024

Mosquitocidal toxin-like islands in Bacillus thuringiensis S2160-1 revealed by complete-genome sequence and MS proteomic analysis.

Yan Zhou, Wenfei Zhang, Yusong Wan, Wujun Jin, Yan Zhang, Youzhi Li, Baoshan Chen, Mingguo Jiang, Xuanjun Fang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Decoding the genome of Bacillus thuringiensis subsp. israelensis VCRC B668 to understand its mosquitocidal potential for biopesticide development.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026
    Article
  2. Genomic Insights IntoBioinformatics and biology insights · 2026
    Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yan ZhouGuangxi Key Laboratory for Polysaccharide Materials and Modifications, School of Marine Sciences and Biotechnology, Guangxi Minzu University, Nanning, 530006, China.
Wenfei ZhangMinistry of Education Key Laboratory for Ecology of Tropical Islands, College of Life Sciences, Hainan Normal University, Haikou, 571158, Hainan, China.
Yusong WanBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Wujun JinBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Yan ZhangSchool of Life Science and Technology, Harbin Institute of Technology, Harbin, 150001, China.
Youzhi LiGuangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning, 530004, Guangxi, China.
Baoshan ChenState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Agriculture, Guangxi University, Nanning, 530004, Guangxi, China.
Mingguo JiangGuangxi Key Laboratory for Polysaccharide Materials and Modifications, School of Marine Sciences and Biotechnology, Guangxi Minzu University, Nanning, 530006, China.
Xuanjun FangHainan Institute of Tropical Agricultural Resources, Sanya, 572025, Hainan, China. jim.xj.fang@hitar.org.

Funding

Guangxi Minzu University Introduction of Talent Research Start-up Fund 2020KJQD23Natural Science Foundation of Guangxi Zhuang Autonomous Region 2022GXNSFBA035446The Basic Ability Enhancement Program for Young and Middle-aged Teachers of Guangxi 2022KY0159
6 · The paper itself

Abstract

Here, we present the whole genome sequence of Bt S2160-1, a potential alternative to the mosquitocidal model strain, Bti. One chromosome genome and four mega-plasmids were contained in Bt S2160-1, and 13 predicted genes encoding predicted insecticidal crystal proteins were identified clustered on one plasmid pS2160-1p2 containing two pathogenic islands (PAIs) designed as PAI-1 (Cry54Ba, Cry30Ea4, Cry69Aa-like, Cry50Ba2-like, Cry4Ca1-like, Cry30Ga2, Cry71Aa-like, Cry72Aa-like, Cry70Aa-like, Cyt1Da2-like and Vpb4C1-like) and PAI-2 (Cyt1Aa-like, and Tpp80Aa1-like). The clusters appear to represent mosquitocidal toxin islands similar to pathogenicity islands. Transcription/translation of 10 of the 13 predicted genes was confirmed by whole-proteome analysis using LTQ-Orbitrap LC-MS/MS. In summary, the present study identified the existence of a mosquitocidal toxin island in Bacillus thuringiensis, and provides important genomic information for understanding the insecticidal mechanism of B. thuringiensis.

Indexed as

Bacillus thuringiensisBacterial ProteinsInsecticidesProteomicsAnimalsBacillus thuringiensis ToxinsEndotoxinsGenome, BacterialGenomic IslandsHemolysin ProteinsPlasmidsProteomeTandem Mass SpectrometryWhole Genome SequencingBacillus thuringiensis ToxinsBacterial ProteinsEndotoxinsHemolysin Proteinsinsecticidal crystal protein, Bacillus ThuringiensisInsecticidesProteomeBacillus thuringiensisBt S2160-1Complete-genome sequenceLTQ-Orbitrap LC–MS/MSMosquitocidal toxin-like island

Identifiers

PMID38956138
PMCPMC11219804

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