ArticleCell discovery2024
Pig blastocyst-like structure models from embryonic stem cells.
Article in Cell discovery, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- Livestock Blastoids: In Vitro Blastocyst Models for Embryogenesis and Reproductive Technologies.Reproduction in domestic animals = Zuchthygiene · 2026Review
- The making of cells and organs in xenogeneic animal bioreactor.Signal transduction and targeted therapy · 2026Review
- Generation of Functional GnRH-Responsive Pituitary-like Organoids from Ovine Embryonic Stem Cells.Cells · 2026Article
- Article
- Sheep blastocyst-like structure models derived from stem cells.Cell discovery · 2026Article
- Generating high-quality porcine iPSCs with the new medium cocktail LACID.Stem cell reports · 2026Article
- Amniogenesis in embryos and stem cell models.Nature cell biology · 2026Review
- In vitro mimicking of humanized cardiogenesis under porcine condition.Cell & bioscience · 2026Article
- Advances in porcine stem cell research and their applications in agriculture.Frontiers in cell and developmental biology · 2026Review
- BMP4 and FGF2 promote heterogeneous priming including extraembryonic lineages in porcine expanded potential stem cells.Frontiers in cell and developmental biology · 2026Article
- Modelling mammalian implantationAnimal reproduction · 2026Review
- Generation of Porcine Blastoids from Embryonic Stem Cells.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Stem Cell-Derived Embryo Models: Potential Platforms for Investigating Early Development and Embryotoxicity.International journal of stem cells · 2025Review
- Steroid hormone-treated endometrial organoids enhance implantation ofJournal of veterinary science · 2025Article
- CRISPR Screening Reveals a Novel Role for FOXH1 in Regulating Pluripotency of Porcine Embryonic Stem Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Stem cell-based embryo models: a tool to study early human development.Science China. Life sciences · 2025Review
- Application of induced pluripotent stem cells in the conservation of endangered animals.Stem cell research & therapy · 2025Review
- Wnt Inhibition Safeguards Porcine Embryonic Stem Cells From the Acquisition of Extraembryonic Endoderm Cell Fates.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Interleukin-7 promotes porcine early embryogenesis in vitro and inner cell mass development through PI3K/AKT pathway after parthenogenetic activation.Scientific reports · 2025Article
- Current Status of Synthetic Mammalian Embryo Models.International journal of molecular sciences · 2024Review
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Pluripotent stem cells have the potential to generate embryo models that can recapitulate developmental processes in vitro. Large animals such as pigs may also benefit from stem-cell-based embryo models for improving breeding. Here, we report the generation of blastoids from porcine embryonic stem cells (pESCs). We first develop a culture medium 4FIXY to derive pESCs. We develop a 3D two-step differentiation strategy to generate porcine blastoids from the pESCs. The resulting blastoids exhibit similar morphology, size, cell lineage composition, and single-cell transcriptome characteristics to blastocysts. These porcine blastoids survive and expand for more than two weeks in vitro under two different culture conditions. Large animal blastoids such as those derived from pESCs may enable in vitro modeling of early embryogenesis and improve livestock species' breeding practices.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.