ArticleThe AAPS journal2024
The Influence of Various Freezing-thawing Methods of Skin on Drug Permeation and Skin Barrier Function.
Article in The AAPS journal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Cracking the Skin Barrier: Models and Methods Driving Dermal Drug Delivery.Pharmaceutics · 2025Review
- Mechanistic Insights Underlying the Drug Release and Skin Permeation of Guanfacine Transdermal Patch with Various Acrylic Pressure-Sensitive Adhesives.AAPS PharmSciTech · 2025Article
- A Chinese drug-compatibility-based approach to purslane hydrogels for acute eczema therapy.Frontiers in pharmacology · 2025Article
- Nanotechnology-driven nanoemulsion gel for enhanced transdermal delivery ofFrontiers in bioengineering and biotechnology · 2025Article
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Authors and funding
6 authors.
Funding
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Abstract
The selection of skin is crucial for the in vitro permeation test (IVPT). The purpose of this study was to investigate the influence of different freezing-thawing processes on the barrier function of skin and the transdermal permeability of granisetron and lidocaine. Rat and hairless mouse skins were thawed at three different conditions after being frozen at -20℃ for 9 days: thawed at 4℃, room temperature (RT), and 32℃. There were no significant differences in the steady-state fluxes of drugs between fresh and thawed samples, but compared with fresh skin there were significant differences in lag time for the permeation of granisetron in rat skins thawed at RT and 32℃. Histological research and scanning electron microscopy images showed no obvious structural damage on frozen/thawed skin, while immunohistochemical staining and enzyme-linked immunosorbent assay for the tight junction (TJ) protein Cldn-1 showed significantly impaired epidermal barrier. It was concluded that the freezing-thawing process increases the diffusion rate of hydrophilic drugs partly due to the functional degradation of TJs. It's recommended that hairless, inbred strains and identical animal donors should be used, and the selected thawing method of skin should be validated prior to IVPT, especially for hydrophilic drugs.
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Registered trials
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