Evaluating the Activity of Ras/Rap GTPases in Dictyostelium.

Stephen F Smith, Pascale G Charest

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

2 authors.

Stephen F SmithDepartment of Chemistry and Biochemistry, University of Arizona, Tucson, AZ, USA.

Pascale G CharestDepartment of Chemistry and Biochemistry, University of Arizona, Tucson, AZ, USA. pcharest@arizona.edu.

Funding

CHEMISTRY-BIOLOGY INTERFACE TRAINING PROGRAMT32GM008804 · NIGMS · UNIVERSITY OF ARIZONA · PI MONTFORT, WILLIAM R. · 2003 to 2023

$3.9M

The role and regulation of mTORC2 in cell migrationR01GM131200 · NIGMS · UNIVERSITY OF ARIZONA · PI CHAREST, PASCALE G · 2019 to 2023

$1.6M

NIGMS NIH HHS R01 GM131200NIGMS NIH HHS T32 GM008804

6 · The paper itself

Abstract

Ras and Rap small GTPases of the Ras superfamily act as molecular switches to control diverse cellular processes as part of different signaling pathways. Dictyostelium expresses several Ras and Rap proteins, and their study has and continues to greatly contribute to our understanding of their role in eukaryote biology. To study the activity of Ras and Rap proteins in Dictyostelium, several assays based on their interaction with the Ras binding domain of known eukaryotic Ras/Rap effectors have been developed and proved extremely useful to study their regulation and cellular roles. Here, we describe methods to assess Ras/Rap activity biochemically using a pull-down assay and through live-cell imaging using fluorescent reporters.

Indexed as

Dictyosteliumras ProteinsProtein BindingProtozoan Proteinsrap GTP-Binding ProteinsSignal TransductionProtozoan Proteinsrap GTP-Binding Proteinsras ProteinsActivity assaysDictyostelium discoideumImagingPull-downRap1RasRas binding domain

Identifiers

PMID38954205

DOI10.1242/jcs.177170

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.