Evidence map›Paper›PMID 38954194›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Discovery and Quantitative Dissection of Cytokinesis Mechanisms Using Dictyostelium discoideum.

Jennifer M K Nguyen, Yinan Liu, Ly Nguyen, Venkataramana K Sidhaye, Douglas N Robinson

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Jennifer M K NguyenDepartment of Cell Biology, Johns Hopkins School of Medicine, Baltimore, MD, USA.
Yinan LiuDepartment of Cell Biology, Johns Hopkins School of Medicine, Baltimore, MD, USA.
Ly NguyenDepartment of Cell Biology, Johns Hopkins School of Medicine, Baltimore, MD, USA.
Venkataramana K SidhayeDepartment of Medicine, Johns Hopkins School of Medicine, Baltimore, MD, USA.
Douglas N RobinsonDepartment of Cell Biology, Johns Hopkins School of Medicine, Baltimore, MD, USA. dnr@jhmi.edu.

Funding

The Biochemical Basis for the Mechanics of CytokinesisR01GM066817 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI ROBINSON, DOUGLAS N · 2003 to 2025
$7.3M
Sickle Cell Disease Associated with Pulmonary Hypertension: Mechanical and Hemodynamic ConsequencesF31HL128088 · NHLBI · UNIVERSITY OF WISCONSIN-MADISON · PI SCHREIER, DAVID A. · 2015 to 2019
$137k
Characterization of Adenine Nucleotide Translocase (ANT) and Actin-Interacting Protein 1 (AIP1) as Protectors Against Cigarette SmokeF31HL145910 · NHLBI · JOHNS HOPKINS UNIVERSITY · PI NGUYEN, JENNIFER · 2019 to 2021
$111k
DARPA HR0011-16-C-0139NHLBI NIH HHS F31 HL128088NHLBI NIH HHS F31 HL145910NIGMS NIH HHS R01 GM066817
6 · The paper itself

Abstract

The social amoeba Dictyostelium discoideum is a versatile model for understanding many different cellular processes involving cell motility including chemotaxis, phagocytosis, and cytokinesis. Cytokinesis, in particular, is a model cell-shaped change process in which a cell separates into two daughter cells. D. discoideum has been used extensively to identify players in cytokinesis and understand how they comprise the mechanosensory and biochemical pathways of cytokinesis. In this chapter, we describe how we use cDNA library complementation with D. discoideum to discover potential regulators of cytokinesis. Once identified, these regulators are further analyzed through live cell imaging, immunofluorescence imaging, fluorescence correlation and cross-correlation spectroscopy, micropipette aspiration, and fluorescence recovery after photobleaching. Collectively, these methods aid in detailing the mechanisms and signaling pathways that comprise cell division.

Indexed as

CytokinesisDictyosteliumFluorescence Recovery After PhotobleachingGene LibraryProtozoan ProteinsSignal TransductionProtozoan ProteinscDNA library complementationCytoskeletal networkDictyostelium discoideumMechanoresponsiveProtein dynamics

Identifiers

PMID38954194

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.