Evidence map›Paper›PMID 38951796›Full record

ArticleBMC genomics2024

Unexpected regulatory functions of cyprinid Viperin on inflammation and metabolism.

Lise Chaumont, Luc Jouneau, François Huetz, Doret R van Muilekom, Mathilde Peruzzi, Claudine Raffy, Jérôme Le Hir, Jules Minke, Pierre Boudinot, Bertrand Collet

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Lise ChaumontUniversité Paris-Saclay, INRAE, UVSQ, VIM, 78350, Jouy-en-Josas, France.
Luc JouneauUniversité Paris-Saclay, INRAE, UVSQ, VIM, 78350, Jouy-en-Josas, France.
François HuetzUnit of Antibodies in Therapy and Pathology, UMR 1222 INSERM, Institut Pasteur, 75015, Paris, France.
Doret R van MuilekomUniversité Paris-Saclay, INRAE, UVSQ, VIM, 78350, Jouy-en-Josas, France.
Mathilde PeruzziUniversité Paris-Saclay, INRAE, UVSQ, VIM, 78350, Jouy-en-Josas, France.
Claudine RaffyVIRBAC S.A, 06510, Carros, France.
Jérôme Le HirVIRBAC S.A, 06510, Carros, France.
Jules MinkeVIRBAC S.A, 06510, Carros, France.
Pierre BoudinotUniversité Paris-Saclay, INRAE, UVSQ, VIM, 78350, Jouy-en-Josas, France.
Bertrand ColletUniversité Paris-Saclay, INRAE, UVSQ, VIM, 78350, Jouy-en-Josas, France. bertrand.collet@inrae.fr.

Funding

Association Nationale de la Recherche et de la Technologie #2020/0646European Commission 871108Norges Forskningsråd 301083
6 · The paper itself

Abstract

backgroundViperin, also known as radical S-adenosyl-methionine domain containing protein 2 (RSAD2), is an interferon-inducible protein that is involved in the innate immune response against a wide array of viruses. In mammals, Viperin exerts its antiviral function through enzymatic conversion of cytidine triphosphate (CTP) into its antiviral analog ddhCTP as well as through interactions with host proteins involved in innate immune signaling and in metabolic pathways exploited by viruses during their life cycle. However, how Viperin modulates the antiviral response in fish remains largely unknown.

resultsFor this purpose, we developed a fathead minnow (Pimephales promelas) clonal cell line in which the unique viperin gene has been knocked out by CRISPR/Cas9 genome-editing. In order to decipher the contribution of fish Viperin to the antiviral response and its regulatory role beyond the scope of the innate immune response, we performed a comparative RNA-seq analysis of viperin

conclusionsOur results revealed that Viperin does not exert positive feedback on the canonical type I IFN but acts as a negative regulator of the inflammatory response by downregulating specific pro-inflammatory genes and upregulating repressors of the NF-κB pathway. It also appeared to play a role in regulating metabolic processes, including one carbon metabolism, bone formation, extracellular matrix organization and cell adhesion.

Indexed as

CyprinidaeInflammationAnimalsCell LineCRISPR-Cas SystemsFish ProteinsGene EditingGene Expression RegulationImmunity, InnateInterferon Type IFish ProteinsInterferon Type ICell adhesionExtracellular matrixFathead minnowInflammatory responseInterferon responseOne carbon metabolismPimephales promelasRNA-SeqRSAD2Viperin

Identifiers

PMID38951796
PMCPMC11218377

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.