Evidence map›Paper›PMID 38951511›Full record

ArticleScientific reports2024

sUPRa is a dual-color reporter for unbiased quantification of the unfolded protein response with cellular resolution.

Atreyi Chakrabarty, Sarah E Newey, Maisha M Promi, Belinda K Agbetiameh, Daniella Munro, Paul J N Brodersen, Gemma Gothard, Kashif Mahfooz, Jose P Mengual, Vladyslav V Vyazovskiy and 1 more

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Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Atreyi ChakrabartyDepartment of Pharmacology, University of Oxford, University of Oxford, Mansfield Road, Oxford, OX1 3QT, UK.
Sarah E NeweyDepartment of Pharmacology, University of Oxford, University of Oxford, Mansfield Road, Oxford, OX1 3QT, UK.
Maisha M PromiDepartment of Pharmacology, University of Oxford, University of Oxford, Mansfield Road, Oxford, OX1 3QT, UK.
Belinda K AgbetiamehDepartment of Pharmacology, University of Oxford, University of Oxford, Mansfield Road, Oxford, OX1 3QT, UK.
Daniella MunroDepartment of Pharmacology, University of Oxford, University of Oxford, Mansfield Road, Oxford, OX1 3QT, UK.
Paul J N BrodersenDepartment of Pharmacology, University of Oxford, University of Oxford, Mansfield Road, Oxford, OX1 3QT, UK.
Gemma GothardDepartment of Pharmacology, University of Oxford, University of Oxford, Mansfield Road, Oxford, OX1 3QT, UK.
Kashif MahfoozDepartment of Pharmacology, University of Oxford, University of Oxford, Mansfield Road, Oxford, OX1 3QT, UK.
Jose P MengualDepartment of Physiology, Anatomy and Genetics, University of Oxford, Sherrington Building, Sherrington Road, Oxford, OX1 3PT, UK.
Vladyslav V VyazovskiyDepartment of Physiology, Anatomy and Genetics, University of Oxford, Sherrington Building, Sherrington Road, Oxford, OX1 3PT, UK.
Colin J AkermanDepartment of Pharmacology, University of Oxford, University of Oxford, Mansfield Road, Oxford, OX1 3QT, UK. colin.akerman@pharm.ox.ac.uk.

Funding

Biotechnology and Biological Sciences Research Council BB/M011224/1Biotechnology and Biological Sciences Research Council BB/S007938/1HORIZON EUROPE European Research Council 617670Leverhulme Trust RPG-2023-083Medical Research Council MR/S01134X/1Wellcome Trust
6 · The paper itself

Abstract

The unfolded protein response (UPR) maintains proteostasis upon endoplasmic reticulum (ER) stress, and is initiated by a range of physiological and pathological processes. While there have been advances in developing fluorescent reporters for monitoring individual signaling pathways of the UPR, this approach may not capture a cell's overall UPR activity. Here we describe a novel sensor of UPR activity, sUPRa, which is designed to report the global UPR. sUPRa displays excellent response characteristics, outperforms reporters of individual UPR pathways in terms of sensitivity and kinetics, and responds to a range of different ER stress stimuli. Furthermore, sUPRa's dual promoter and fluorescent protein design ensures that both UPR-active and inactive cells are detected, and controls for reporter copy number. Using sUPRa, we reveal UPR activation in layer 2/3 pyramidal neurons of mouse cerebral cortex following a period of sleep deprivation. sUPRa affords new opportunities for quantifying physiological UPR activity with cellular resolution.

Indexed as

Endoplasmic Reticulum StressUnfolded Protein ResponseAnimalsGenes, ReporterHumansLuminescent ProteinsMicePyramidal CellsSignal TransductionLuminescent Proteins

Identifiers

PMID38951511
PMCPMC11217371

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.