Evidence map›Paper›PMID 38951345›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Nonviral Platform for Expression of Recombinant Protein in Insect Cells.

Jagadeesh Mahadevan, Kishalay Mitra, Lopamudra Giri

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Jagadeesh MahadevanDepartment of Chemical Engineering, Indian Institute of Technology, Hyderabad, India.
Kishalay MitraDepartment of Chemical Engineering, Indian Institute of Technology, Hyderabad, India.
Lopamudra GiriDepartment of Chemical Engineering, Indian Institute of Technology, Hyderabad, India. Giril@che.iith.ac.in.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Nonviral transfection has been used to express various recombinant proteins, therapeutics, and virus-like particles (VLP) in mammalian and insect cells. Virus-free methods for protein expression require fewer steps for obtaining protein expression by eliminating virus amplification and measuring the infectivity of the virus. The nonviral method uses a nonlytic plasmid to transfect the gene of interest into the insect cells instead of using baculovirus, a lytic system. In this chapter, we describe one of the transfection methods, which uses polyethyleneimine (PEI) as a DNA delivery material into the insect cells to express the recombinant protein in both adherent and suspension cells.

Indexed as

PolyethyleneimineRecombinant ProteinsTransfectionAnimalsCell LineGene ExpressionInsectaPlasmidsSf9 CellsSpodopteraPolyethyleneimineRecombinant ProteinsBaculovirus free expressionInsect cellsNonviral protein expressionPEI mediated transfectionRecombinant proteinTransient transfectionVLPs

Identifiers

PMID38951345

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.