Evidence map›Paper›PMID 38951333›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Production of Secreted Antibody in Baculovirus Expression Vector System.

Zhihui Zhang, Xiaoyue Zhang, Lulu Liu, Hongying Chen

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Zhihui ZhangCollege of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China.
Xiaoyue ZhangCollege of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China.
Lulu LiuCollege of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China.
Hongying ChenCollege of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China. chenhy@nwafu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Monoclonal antibodies have widespread applications in disease treatment and antigen detection. They are traditionally produced using mammalian cell expression system, which is not able to satisfy the increasing demand of these proteins at large scale. Baculovirus expression vector system (BEVS) is an attractive alternative platform for the production of biologically active monoclonal antibodies. In this chapter, we demonstrate the production of an HIV-1 broadly neutralizing antibody b12 in BEVS. The processes including transfer vector construction, recombinant baculovirus generation, and antibody production and detection are described.

Indexed as

BaculoviridaeGenetic VectorsAnimalsAntibodies, MonoclonalAntibodies, NeutralizingEnzyme-Linked Immunosorbent AssayGene ExpressionHIV-1HIV AntibodiesHumansRecombinant ProteinsSf9 CellsAntibodies, MonoclonalAntibodies, NeutralizingHIV AntibodiesRecombinant ProteinsAcMNPVAntibodyb12Baculovirus expression vector systemCo-expressionCo-infectionProtein expression

Identifiers

PMID38951333

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.