Evidence map›Paper›PMID 38951263›Full record

ArticleMikrochimica acta2024

One-pot electrochemical detection of foodborne pathogen based on in situ nucleic acid amplification and wash-free assay.

Xinyi Li, Yaofang Fan, Hao Gong, Haoran Wang, Yanli Ji, Longqiang Xu, Cuiping Ma, Chao Shi

Abstract read
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In one paragraph

Article in Mikrochimica acta, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Xinyi Li *
Yaofang Fan *
Hao GongQingdao Nucleic Acid Rapid Testing International Science and Technology Cooperation Base, College of Life SciencesDepartment of Pathogenic Biology, School of Basic MedicineDepartment of Clinical Laboratory, the Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, 266071, People's Republic of China.
Haoran WangShandong Provincial Key Laboratory of Biochemical Engineering, Qingdao Key Laboratory of Nucleic Acid Rapid Detection, Sino-UAE International Cooperative Joint Laboratory of Pathogenic Microorganism Rapid Detection, College of Biological Engineering, Qingdao Nucleic Acid Rapid Detection Engineering Research Center, Qingdao University of Science and Technology, Qingdao, 266042, People's Republic of China.
Yanli JiQingdao JianMa Gene Technology Co., Ltd, Qingdao, 266114, People's Republic of China.
Longqiang XuDepartment of Clinical Laboratory, The Affiliated Hospital of Qingdao University, Qingdao, 266071, People's Republic of China. xulongqiang75@sina.com.
Cuiping MaShandong Provincial Key Laboratory of Biochemical Engineering, Qingdao Key Laboratory of Nucleic Acid Rapid Detection, Sino-UAE International Cooperative Joint Laboratory of Pathogenic Microorganism Rapid Detection, College of Biological Engineering, Qingdao Nucleic Acid Rapid Detection Engineering Research Center, Qingdao University of Science and Technology, Qingdao, 266042, People's Republic of China.
Chao ShiQingdao Nucleic Acid Rapid Testing International Science and Technology Cooperation Base, College of Life SciencesDepartment of Pathogenic Biology, School of Basic MedicineDepartment of Clinical Laboratory, the Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, 266071, People's Republic of China. sc169@163.com.

Funding

Key Project of Shandong Province Natural Science Foundation ZR2020KH030Science and Technology Benefiting the People Demonstration Project of Qingdao 22-2-7-smjk-2-nsh
6 · The paper itself

Abstract

A signal amplification electrochemical biosensor chip was developed to integrate loop-mediated isothermal amplification (LAMP) based on in situ nucleic acid amplification and methyl blue (MB) serving as the hybridization redox indicator for sensitive and selective foodborne pathogen detection without a washing step. The electrochemical biosensor chip was designed by a screen-printed carbon electrode modified with gold nanoparticles (Au NPs) and covered with polydimethylsiloxane membrane to form a microcell. The primers of the target were immobilized on the Au NPs by covalent attachment for in situ amplification. The electroactive MB was used as the electrochemical signal reporter and embedded into the double-stranded DNA (dsDNA) amplicons generated by LAMP. Differential pulse voltammetry was introduced to survey the dsDNA hybridization with MB, which differentiates the specifically electrode-unbound and -bound labels without a washing step. Pyrene as the back-filling agent can further improve response signaling by reducing non-specific adsorption. This method is operationally simple, specific, and effective. The biosensor showed a detection linear range of 10

Indexed as

Biosensing TechniquesElectrochemical TechniquesFood MicrobiologyGoldMetal NanoparticlesNucleic Acid Amplification TechniquesDNA, BacterialElectrodesLimit of DetectionNucleic Acid HybridizationDNA, BacterialGoldElectrochemical biosensorFood pathogens detectionLoop-mediated isothermal amplificationMethylene blueScreen printed carbon electrode

Identifiers

PMID38951263

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.