Evidence map›Paper›PMID 38951211›Full record

ArticleMycorrhiza2024

An updated LSU database and pipeline for environmental DNA identification of arbuscular mycorrhizal fungi.

Camille S Delavaux, Robert J Ramos, Sidney L Stürmer, James D Bever

Abstract read
In one paragraph

Article in Mycorrhiza, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Camille S Delavaux *Institute of Integrative Biology, ETH Zurich (Swiss Federal Institute of Technology), Universitätsstrasse 16, Zurich, 8092, Switzerland. camille.delavaux@usys.ethz.ch.ORCID http://orcid.org/0000-0002-8340-2173
Robert J Ramos *The Environmental Data Science Innovation & Inclusion Lab (ESIIL), University of Colorado Boulder, Colorado, 80309, USA.ORCID http://orcid.org/0000-0002-5545-702X
Sidney L StürmerDepartamento de Ciências Naturais, Universidade Regional de Blumenau, Blumenau, Santa Catarina, 89030-903, Brazil.ORCID http://orcid.org/0000-0003-3213-1841
James D BeverDepartment of Ecology and Evolutionary Biology, The University of Kansas, 2041 Haworth Hall, 1200 Sunnyside Avenue, Lawrence, KS, 66045, USA.ORCID http://orcid.org/0000-0003-4068-3582

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Recent work established a backbone reference tree and phylogenetic placement pipeline for identification of arbuscular mycorrhizal fungal (AMF) large subunit (LSU) rDNA environmental sequences. Our previously published pipeline allowed any environmental sequence to be identified as putative AMF or within one of the major families. Despite this contribution, difficulties in implementation of the pipeline remain. Here, we present an updated database and pipeline with (1) an expanded backbone tree to include four newly described genera and (2) several changes to improve ease and consistency of implementation. In particular, packages required for the pipeline are now installed as a single folder (conda environment) and the pipeline has been tested across three university computing clusters. This updated backbone tree and pipeline will enable broadened adoption by the community, advancing our understanding of these ubiquitous and ecologically important fungi.

Indexed as

DNA, FungalMycorrhizaePhylogenyDNA, EnvironmentalDNA, RibosomalSoil MicrobiologyDNA, EnvironmentalDNA, FungalDNA, RibosomalArbuscular mycorrhizal fungiEnvironmental DNA sequencesIllumina sequencingLarge subunit rDNAPhylogenetics

Identifiers

PMID38951211
PMCPMC11283431

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.