Evidence map›Paper›PMID 38940971›Full record

ArticleDigestive diseases and sciences2024

Rs12039395 Variant Influences the Expression of hsa-miR-181a-5p and PTEN Toward Colorectal Cancer Risk.

Wael A El-Korany, Walid E Zahran, Mohamed A Alm El-Din, Hanan A Al-Shenawy, Ahmed F Soliman

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Article in Digestive diseases and sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.

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1citing papers in PubMed
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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Expert opinion on therapeutic targets · 2024
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Wael A El-KoranyBiochemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt.
Walid E ZahranBiochemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt.
Mohamed A Alm El-DinClinical Oncology Department, Faculty of Medicine, Tanta University, Gharbia, Egypt.
Hanan A Al-ShenawyPathology Department, Faculty of Medicine, Tanta University, Gharbia, Egypt.
Ahmed F SolimanBiochemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt. ahmed.fathi@sci.asu.edu.eg.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundSingle nucleotide polymorphisms (SNPs) in microRNA (miRNA) genes could alter miRNA expression levels or processing and, thus, may contribute to colorectal cancer (CRC) development. Therefore, this study aimed to examine whether the MIR181A1 genomic sequence possesses SNPs that can affect the expression of hsa-miR-181a-5p and, subsequently, impact its targets and associate with CRC risk.

methodsThe NCBI dbSNP database was searched for possible SNPs associated with MIR181A1. One SNP with a minor allele frequency > 5%, rs12039395 G > T was identified. In silico analyses determined the effect of the SNP on the secondary structure of the miRNA and predicted the hsa-miR-181a-5p target genes. The SNP was genotyped using allelic discrimination assay, the relative hsa-miR-181a-5p expression level was determined using quantitative real-time PCR, and immunohistochemical staining was used to detect target genes in 192 paraffin-embedded specimens collected from 160 CRC patients and 32 healthy subjects.

resultsThe rs6505162 SNP conferred protection against CRC, and the G-allele presence provides may provide accessibility for the transcriptional machinery. Hsa-miR-181a-5p was significantly over-expressed in the CRC group compared to controls and in samples carrying the G-allele compared to those with T-allele. PTEN, identified as the only hsa-miR-181a-5p target implicated in CRC, was significantly diminished in the CRC group compared to controls and showed an inverse relationship with hsa-miR-181a-5p expression level as well as negatively associated with the G-allele presence in CRC.

conclusionThis study highlights that rs12039395 G > T may protect against CRC by influencing the expression of hsa-mir-181a-5p and its target gene, PTEN.

Indexed as

Colorectal NeoplasmsGenetic Predisposition to DiseaseMicroRNAsPolymorphism, Single NucleotidePTEN PhosphohydrolaseAgedCase-Control StudiesFemaleGene Expression Regulation, NeoplasticHumansMaleMiddle AgedMicroRNAsMIrn181 microRNA, humanPTEN PhosphohydrolasePTEN protein, humanColorectal cancer (CRC)miR-181aPhosphatase and tensin homolog (PTEN)Single nucleotide polymorphism (SNP)

Identifiers

PMID38940971

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