Evidence map›Paper›PMID 38940473›Full record

ArticleDevelopment (Cambridge, England)2024

Natural reversal of cavefish heart asymmetry is controlled by Sonic Hedgehog effects on the left-right organizer.

Mandy Ng, Li Ma, Janet Shi, William R Jeffery

Abstract read
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In one paragraph

Article in Development (Cambridge, England), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Mandy NgDepartment of Biology, University of Maryland, College Park, MD 20742, USA.ORCID 0000-0001-8267-8330
Li MaDepartment of Biology, University of Maryland, College Park, MD 20742, USA.
Janet ShiDepartment of Biology, University of Maryland, College Park, MD 20742, USA.ORCID 0000-0002-1125-8133
William R JefferyDepartment of Biology, University of Maryland, College Park, MD 20742, USA.ORCID 0000-0002-6997-2946

Funding

Molecular Genetic Analysis of Sclera Development and DegenerationR01EY024941 · NEI · UNIV OF MARYLAND, COLLEGE PARK · PI JEFFERY, WILLIAM R · 2015 to 2019
$1.9M
Zeiss LSM 880 Airyscan Fast Laser Scanning ConfocalS10OD025223 · OD · UNIV OF MARYLAND, COLLEGE PARK · PI BEAVEN, AMY · 2019 to 2019
$557k
NEI NIH HHS R01 EY024941NIH HHS EY024941NIH HHS S10 OD025223University of Maryland, College Park
6 · The paper itself

Abstract

The direction of left-right visceral asymmetry is conserved in vertebrates. Deviations of the standard asymmetric pattern are rare, and the underlying mechanisms are not understood. Here, we use the teleost Astyanax mexicanus, consisting of surface fish with normal left-oriented heart asymmetry and cavefish with high levels of reversed right-oriented heart asymmetry, to explore natural changes in asymmetry determination. We show that Sonic Hedgehog (Shh) signaling is increased at the posterior midline, Kupffer's vesicle (the teleost left-right organizer) is enlarged and contains longer cilia, and the number of dorsal forerunner cells is increased in cavefish. Furthermore, Shh increase in surface fish embryos induces asymmetric changes resembling the cavefish phenotype. Asymmetric expression of the Nodal antagonist Dand5 is equalized or reversed in cavefish, and Shh increase in surface fish mimics changes in cavefish dand5 asymmetry. Shh decrease reduces the level of right-oriented heart asymmetry in cavefish. Thus, naturally occurring modifications in cavefish heart asymmetry are controlled by the effects of Shh signaling on left-right organizer function.

Indexed as

Body PatterningHeartHedgehog ProteinsSignal TransductionAnimalsCharacidaeCiliaEmbryo, NonmammalianGene Expression Regulation, DevelopmentalHedgehog ProteinsCavefishdand5 asymmetryHeart asymmetryLeft-right organizerSonic Hedgehog

Identifiers

PMID38940473
PMCPMC11273321

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.