ArticleJournal of extracellular biology2023
Endothelial cells release microvesicles that harbour multivesicular bodies and secrete exosomes.
Article in Journal of extracellular biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed.
- Nano-Engineered Titanium Implants Loaded With Gingival Fibroblasts-Derived Microvesicles Enhance Early Osseointegration And Soft Tissue Attachment In Vivo.Advanced healthcare materials · 2026Article
- Simulation of intracellular delivery through permeabilized multivesicular vesicles.European biophysics journal : EBJ · 2026Article
- Roles and Potential Mechanisms of Endothelial Cell-Derived Extracellular Vesicles in Ischemic Stroke.Translational stroke research · 2025Review
- Multiomic Landscape of Extracellular Vesicles in Human Carotid Atherosclerotic Plaque Reveals Endothelial Communication Networks.Arteriosclerosis, thrombosis, and vascular biology · 2025Article
- Phospholipid-Rich DC-Vesicles with Preserved Immune Fingerprints: A Stable and Scalable Platform for Precision Immunotherapy.Biomedicines · 2025Article
- A class of large cell-like extracellular vesicles.Nature cell biology · 2025Article
- Blebbisomes are large, organelle-rich extracellular vesicles with cell-like properties.Nature cell biology · 2025Article
- Deciphering Platelets: Are They Cells or an Evolved Form of Extracellular Vesicles?Circulation research · 2025Review
- Article
- Diverse-Origin Exosomes Therapeutic Strategies for Diabetic Wound Healing.International journal of nanomedicine · 2025Review
- Tau filaments are tethered within brain extracellular vesicles in Alzheimer's disease.Nature neuroscience · 2025Article
- Immune System-Related Plasma Pathogenic Extracellular Vesicle Subpopulations Predict Osteoarthritis Progression.International journal of molecular sciences · 2024Article
- Activation of the receptor KIT induces the secretion of exosome-like small extracellular vesicles.Journal of extracellular biology · 2024Article
- Relevance of multilamellar and multicompartmental vesicles in biological fluids: understanding the significance of proportional variations and disease correlation.Biomarker research · 2023Review
- Extracellular Vesicles and Intercellular Communication: Challenges for In Vivo Molecular Imaging and Tracking.Pharmaceutics · 2023Review
- Endothelial cells release microvesicles that harbour multivesicular bodies and secrete exosomes.Journal of extracellular biology · 2023Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Extracellular vesicles (EVs) released by endothelial cells support vascular homeostasis. To better understand endothelial cell EV biogenesis, we examined cultured human umbilical vein endothelial cells (HUVECs) prepared by rapid freezing, freeze-substitution, and serial thin section electron microscopy (EM). Thin sections of HUVECs revealed clusters of membrane protrusions on the otherwise smooth cell surface. The protrusions contained membrane-bound organelles, including multivesicular bodies (MVBs), and appeared to be on the verge of pinching off to form microvesicles. Beyond cell peripheries, membrane-bound vesicles with internal MVBs were observed, and serial sections confirmed that they were not connected to cells. These observations are consistent with the notion that these multi-compartmented microvesicles (MCMVs) pinch-off from protrusions. Remarkably, omega figures formed by fusion of vesicles with the MCMV limiting membrane were directly observed, apparently releasing exosomes from the MCMV. In summary, MCMVs are a novel form of EV that bud from membrane protrusions on the HUVEC surface, contain MVBs and release exosomes. These observations suggest that exosomes can be harboured within and released from transiting microvesicles after departure from the parent cell, constituting a new site of exosome biogenesis occurring from endothelial and potentially additional cell types.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.