Synthesis of nickel-sphere coated Ni-Mn layer for efficient electrochemical detection of urea.

Using a trustworthy electrochemical sensor in the detection of urea in real blood samples received a great attention these days. A thin layer of nickel-coated nickel-manganese (Ni@NiMn) is electrodeposited on a glassy carbon electrode (GC) (Ni@NiMn/GC) surface and used to construct the electrochemical sensor for urea detection. Whereas, electrodeposition is considered as strong technique for the controllable synthesis of nanoparticles. Thus, X-ray diffraction (XRD), atomic force microscope (AFM), and scanning electron microscope (SEM) techniques were used to characterize the produced electrode. AFM and SEM pictures revealed additional details about the surface morphology, which revealed a homogenous and smooth coating. Furthermore, electrochemical research was carried out in alkaline medium utilizing various electrochemical methods, including cyclic voltammetry (CV), chronoamperometry (CA), and electrochemical impedance spectroscopy (EIS). The electrochemical investigations showed that the electrode had good performance, high stability and effective charge transfer capabilities. The structural, morphological, and electrochemical characteristics of Ni@NiMn/GC electrodes were well understood using the analytical and electrochemical techniques. The electrode showed a limit of detection (LOD) equal to 0.0187 µM and a linear range of detection of 1.0-10 mM of urea. Furthermore, real blood samples were used to examine the efficiency of the prepared sensor. Otherwise, the anti-interfering ability of the modified catalyst was examined toward various interfering species.