ArticlePLoS biology2024
A high-throughput behavioral screening platform for measuring chemotaxis by C. elegans.
Article in PLoS biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed.
- Predation in microbial communities: gradients of nutritive killing.Nature reviews. Microbiology · 2026Review
- TAXISCAN, Optimizing throughput and behavioral depth in standardbioRxiv : the preprint server for biology · 2026Article
- Mechanisms of surface and volume light scattering fromiScience · 2026Article
- Efficient pheromone navigation via antagonistic detectors in Caenorhabditis elegans male.Nature communications · 2026Article
- AI and Microfluidics: Unlocking Cellular Motility for Bioengineering.Bioengineering (Basel, Switzerland) · 2026Review
- Nematicidal indole oxazoles and chemoattractants from soil bacteria.bioRxiv : the preprint server for biology · 2026Article
- Bay leaf extract is a chemotaxis repellent formicroPublication biology · 2026Article
- Tobacco extract is a chemotaxis repellent formicroPublication biology · 2026Article
- Satiety, TAX-4, and OSM-9 tune the attraction of Caenorhabditis elegans nematodes to microbial fermentation products.G3 (Bethesda, Md.) · 2025Article
- The anticonvulsant and mood-stabilizing drug valproic acid attractsbioRxiv : the preprint server for biology · 2025Article
- Article
- Automated Platforms inMicromachines · 2025Review
- High-throughput behavioural phenotyping of 25 C. elegans disease models including patient-specific mutations.BMC biology · 2025Article
- A multigenerational population-growth assay to capture subtle fitness phenotypes in C. elegans and other nematodes.Genetics · 2025Article
- The C. elegans glutamate transporters GLT-4 and GLT-5 regulate protein expression, behavior, and lifespan.Neurochemistry international · 2025Article
- Luminous Upconverted Nanoparticles as High-Sensitivity Optical Probes for Visualizing Nano- and Microplastics inSensors (Basel, Switzerland) · 2025Article
- Utilization of Artificial Intelligence Coupled with a High-Throughput, High-Content Platform in the Exploration of Neurodevelopmental Toxicity of Individual and Combined PFAS.Journal of xenobiotics · 2025Article
- Enhancing Reproducibility in Chemotaxis Assays for Caenorhabditis elegans.Current protocols · 2025Article
- A simple yet reliable assay for chemotaxis inmicroPublication biology · 2025Article
- Velvety tree ant extract is a chemotaxis repellent formicroPublication biology · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
15 authors.
Funding
Abstract
Throughout history, humans have relied on plants as a source of medication, flavoring, and food. Plants synthesize large chemical libraries and release many of these compounds into the rhizosphere and atmosphere where they affect animal and microbe behavior. To survive, nematodes must have evolved the sensory capacity to distinguish plant-made small molecules (SMs) that are harmful and must be avoided from those that are beneficial and should be sought. This ability to classify chemical cues as a function of their value is fundamental to olfaction and represents a capacity shared by many animals, including humans. Here, we present an efficient platform based on multiwell plates, liquid handling instrumentation, inexpensive optical scanners, and bespoke software that can efficiently determine the valence (attraction or repulsion) of single SMs in the model nematode, Caenorhabditis elegans. Using this integrated hardware-wetware-software platform, we screened 90 plant SMs and identified 37 that attracted or repelled wild-type animals but had no effect on mutants defective in chemosensory transduction. Genetic dissection indicates that for at least 10 of these SMs, response valence emerges from the integration of opposing signals, arguing that olfactory valence is often determined by integrating chemosensory signals over multiple lines of information. This study establishes that C. elegans is an effective discovery engine for determining chemotaxis valence and for identifying natural products detected by the chemosensory nervous system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.