Evidence map›Paper›PMID 38935136›Full record

ArticleAmino acids2024

In vitro and in vivo studies on exogenous polyamines and α-difluoromethylornithine to enhance bone formation and suppress osteoclast differentiation.

Chien-Ching Lee, Chia-Chun Chuang, Chung-Hwan Chen, Yuan-Pin Huang, Chiao-Yi Chang, Pei-Yi Tung, Mon-Juan Lee

Abstract read
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In one paragraph

Article in Amino acids, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Chien-Ching Lee *Department of Anesthesia, An Nan Hospital, China Medical University, Tainan, 70965, Taiwan.
Chia-Chun Chuang *Department of Anesthesia, An Nan Hospital, China Medical University, Tainan, 70965, Taiwan.
Chung-Hwan ChenDepartment of Orthopedics, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung, 80145, Taiwan.
Yuan-Pin HuangDepartment of Cosmetics and Fashion Styling, Cheng Shiu University, Kaohsiung, 83347, Taiwan.
Chiao-Yi ChangDepartment of Bioscience Technology, Chang Jung Christian University, Tainan, 711301, Taiwan.
Pei-Yi TungDepartment of Bioscience Technology, Chang Jung Christian University, Tainan, 711301, Taiwan.
Mon-Juan LeeDepartment of Medical Science Industries, Chang Jung Christian University, No.1, Changda Rd., Gueiren District, Tainan, 711301, Taiwan. mjlee@mail.cjcu.edu.tw.

Funding

An Nan Hospital, China Medical University, Tainan, Taiwan ANHRF112-25National Science and Technology Council NSTC 101-2314-B-309-001-MY3
6 · The paper itself

Abstract

Exogenous polyamines, including putrescine (PUT), spermidine (SPD), and spermine (SPM), and the irreversible inhibitor of the rate-limiting enzyme ornithine decarboxylase (ODC) of polyamine biosynthesis, α-difluoromethylornithine (DFMO), are implicated as stimulants for bone formation. We demonstrate in this study the osteogenic potential of exogenous polyamines and DFMO in human osteoblasts (hOBs), murine monocyte cell line RAW 264.7, and an ovariectomized rat model. The effect of polyamines and DFMO on hOBs and RAW 264.7 cells was studied by analyzing gene expression, alkaline phosphatase (ALP) activity, tartrate-resistant acid phosphatase (TRAP) activity, and matrix mineralization. Ovariectomized rats were treated with polyamines and DFMO and analyzed by micro computed tomography (micro CT). The mRNA level of the early onset genes of osteogenic differentiation, Runt-related transcription factor 2 (Runx2) and ALP, was significantly elevated in hOBs under osteogenic conditions, while both ALP activity and matrix mineralization were enhanced by exogenous polyamines and DFMO. Under osteoclastogenic conditions, the gene expression of both receptor activator of nuclear factor-κB (RANK) and nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1) was reduced, and TRAP activity was suppressed by exogenous polyamines and DFMO in RAW 264.7 cells. In an osteoporotic animal model of ovariectomized rats, SPM and DFMO were found to improve bone volume in rat femurs, while trabecular thickness was increased in all treatment groups. Results from this study provide in vitro and in vivo evidence indicating that polyamines and DFMO act as stimulants for bone formation, and their osteogenic effect may be associated with the suppression of osteoclastogenesis.

Indexed as

Cell DifferentiationEflornithineOsteoblastsOsteoclastsOsteogenesisPolyaminesAnimalsFemaleHumansMiceOvariectomyRatsRats, Sprague-DawleyRAW 264.7 CellsSpermidineEflornithinePolyaminesSpermidineOsteoclastogenic differentiationOsteogenic differentiationOsteoporosisOvariectomized ratPolyaminesα-difluoromethylornithine (DFMO)

Identifiers

PMID38935136
PMCPMC11211182

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.