ArticleThe Journal of cell biology2024
Chemical transformation of the multibudding yeast, Aureobasidium pullulans.
Article in The Journal of cell biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed.
- Septins and cytokinesis in the polymorphic fungus Aureobasidium pullulans.The Journal of cell biology · 2026Article
- Organelle scaling over a 100-fold cell size range.bioRxiv : the preprint server for biology · 2026Article
- Organelle partitioning in the multi-budding yeastbioRxiv : the preprint server for biology · 2026Article
- Homology-mediated transformation of frog-killing fungusProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Allocation of resources among multiple daughter cells.The Journal of cell biology · 2025Article
- Mechanisms of nuclear segregation in a multinucleate multibudding yeast.The Journal of cell biology · 2025Article
- A genetic strategy to allow detection of F-actin by phalloidin staining in diverse fungi.mSphere · 2025Article
- Negative feedback equalizes polarity sites in a multi-budding yeast.Current biology : CB · 2025Article
- Optimized vectors for genetic engineering ofMolecular biology of the cell · 2025Article
- Allocation of resources among multiple daughter cells.bioRxiv : the preprint server for biology · 2025Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors.
Funding
Abstract
Aureobasidium pullulans is a ubiquitous polymorphic black yeast with industrial and agricultural applications. It has recently gained attention amongst cell biologists for its unconventional mode of proliferation in which multinucleate yeast cells make multiple buds within a single cell cycle. Here, we combine a chemical transformation method with genome-targeted homologous recombination to yield ∼60 transformants/μg of DNA in just 3 days. This protocol is simple, inexpensive, and requires no specialized equipment. We also describe vectors with codon-optimized green and red fluorescent proteins for A. pullulans and use these tools to explore novel cell biology. Quantitative imaging of a strain expressing cytosolic and nuclear markers showed that although the nuclear number varies considerably among cells of similar volume, total nuclear volume scales with cell volume over an impressive 70-fold size range. The protocols and tools described here expand the toolkit for A. pullulans biologists and will help researchers address the many other puzzles posed by this polyextremotolerant and morphologically plastic organism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.