Evidence map›Paper›PMID 38934530›Full record

ArticleMicrobial biotechnology2024

Controlling the expression of heterologous genes in Bdellovibrio bacteriovorus using synthetic biology strategies.

Sergio Salgado, Natalia Hernández-Herreros, M Auxiliadora Prieto

Abstract read
In one paragraph

Article in Microbial biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Sergio SalgadoInterdisciplinary Platform for Sustainable Plastics towards a Circular Economy-Spanish National Research Council (SusPlast-CSIC), Madrid, Spain.ORCID 0000-0002-5968-465X
Natalia Hernández-HerrerosInterdisciplinary Platform for Sustainable Plastics towards a Circular Economy-Spanish National Research Council (SusPlast-CSIC), Madrid, Spain.ORCID 0000-0002-5416-8971
M Auxiliadora PrietoInterdisciplinary Platform for Sustainable Plastics towards a Circular Economy-Spanish National Research Council (SusPlast-CSIC), Madrid, Spain.ORCID 0000-0002-8038-1223

Funding

Agencia Estatal de Investigación BIO2017-8344-8-RAgencia Estatal de Investigación PID2020-112766RB-C21Comunidad de Madrid P2018/NMT4389Horizon 2020 Framework Programme 76099HORIZON EUROPE Framework Programme 101081776Spanish Ministry of Science, Innovation and Universities FPU17/03978
6 · The paper itself

Abstract

Bdellovibrio bacteriovorus HD100 is an obligate predatory bacterium that preys upon Gram-negative bacteria. It has been proposed to be applied as a "living antibiotic" in several fields such as agriculture or even medicine, since it is able to prey upon bacterial pathogens. Its interesting lifestyle makes this bacterium very attractive as a microbial chassis for co-culture systems including two partners. A limitation to this goal is the scarcity of suitable synthetic biology tools for predator domestication. To fill this gap, we have firstly adapted the hierarchical assembly cloning technique Golden Standard (GS) to make it compatible with B. bacteriovorus HD100. The chromosomal integration of the Tn7 transposon's mobile element, in conjunction with the application of the GS technique, has allowed the systematic characterization of a repertoire of constitutive and inducible promoters, facilitating the control of the expression of heterologous genes in this bacterium. PJ

Indexed as

Bdellovibrio bacteriovorusGene Expression Regulation, BacterialPromoter Regions, GeneticSynthetic BiologyCloning, MolecularDNA Transposable ElementsRecombinant ProteinsDNA Transposable ElementsRecombinant Proteins

Identifiers

PMID38934530
PMCPMC11209729

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.