Evidence map›Paper›PMID 38932257›Full record

ArticleViruses2024

Isolation and Characterization of Contemporary Bovine Coronavirus Strains.

Yu Li, Roberto A Palomares, Mingde Liu, Jiayu Xu, Chohee Koo, Francesca Granberry, Samantha R Locke, Greg Habing, Linda J Saif, Leyi Wang and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Viruses, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Yu LiCenter for Food Animal Health, Department of Animal Sciences, College of Food, Agricultural and Environmental Sciences, The Ohio State University, Wooster, OH 44691, USA.ORCID 0009-0004-1343-1552
Roberto A PalomaresDepartment of Population Health, College of Veterinary Medicine, University of Georgia, 2200 College Station Rd., Athens, GA 30602, USA.ORCID 0000-0002-3390-5098
Mingde LiuCenter for Food Animal Health, Department of Animal Sciences, College of Food, Agricultural and Environmental Sciences, The Ohio State University, Wooster, OH 44691, USA.
Jiayu XuCenter for Food Animal Health, Department of Animal Sciences, College of Food, Agricultural and Environmental Sciences, The Ohio State University, Wooster, OH 44691, USA.
Chohee KooDepartment of Population Health, College of Veterinary Medicine, University of Georgia, 2200 College Station Rd., Athens, GA 30602, USA.
Francesca GranberryDepartment of Population Health, College of Veterinary Medicine, University of Georgia, 2200 College Station Rd., Athens, GA 30602, USA.
Samantha R LockeDepartment of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210, USA.ORCID 0000-0003-1319-0758
Greg HabingDepartment of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210, USA.
Linda J SaifCenter for Food Animal Health, Department of Animal Sciences, College of Food, Agricultural and Environmental Sciences, The Ohio State University, Wooster, OH 44691, USA.ORCID 0000-0002-3224-9009
Leyi WangVeterinary Diagnostic Laboratory, Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Urbana, IL 61802, USA.ORCID 0000-0001-5813-9505
Qiuhong WangCenter for Food Animal Health, Department of Animal Sciences, College of Food, Agricultural and Environmental Sciences, The Ohio State University, Wooster, OH 44691, USA.ORCID 0000-0002-8238-417X

Funding

University of Illinois Veterinary Diagnostic Laboratory participation in Veterinary Laboratory Investigation and Response NetworkU18FD006673 · FDA · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · PI Chien-Che Hung · 2019 to 2026
$387k
FDA HHS U18 FD006673The Ohio State University General FundsUnited States Food and Drug Administration 1U18FD006673
6 · The paper itself

Abstract

Bovine coronavirus (BCoV) poses a threat to cattle health worldwide, contributing to both respiratory and enteric diseases. However, few contemporary strains have been isolated. In this study, 71 samples (10 nasal and 61 fecal) were collected from one farm in Ohio in 2021 and three farms in Georgia in 2023. They were screened by BCoV-specific real-time reverse transcription-PCR, and 15 BCoV-positive samples were identified. Among them, five BCoV strains from fecal samples were isolated using human rectal tumor-18 (HRT-18) cells. The genomic sequences of five strains were obtained. The phylogenetic analysis illustrated that these new strains clustered with US BCoVs that have been detected since the 1990s. Sequence analyses of the spike proteins of four pairs of BCoVs, with each pair originally collected from the respiratory and enteric sites of one animal, revealed the potential amino acid residue patterns, such as D1180 for all four enteric BCoVs and G1180 for three of four respiratory BCoVs. This project provides new BCoV isolates and sequences and underscores the genetic diversity of BcoVs, the unknown mechanisms of disease types, and the necessity of sustained surveillance and research for BCoVs.

Indexed as

Cattle DiseasesCoronavirus, BovineCoronavirus InfectionsFecesPhylogenyAnimalsCattleGenetic VariationGenome, ViralHumansOhioSpike Glycoprotein, CoronavirusSpike Glycoprotein, Coronavirusbovine coronavirusgenomic sequencevirus isolation

Identifiers

PMID38932257
PMCPMC11209117

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.