ArticleMicroorganisms2024
Selection and Multiplexing of Reverse Transcription-Quantitative PCR Tests Targeting Relevant Honeybee Viral Pathogens.
Article in Microorganisms, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Distribution and Quantification of Infectious and Parasitic Agents in Managed Honeybees in Central Italy, the Republic of Kosovo, and Albania.Microorganisms · 2026Article
- Construction of a portable multiplex detection system for four bee viral paralysis diseases based on RT-PCR-microfluidic chip integrated technology.BMC veterinary research · 2025Article
- Use of Hive Debris to Detect Acute Bee Paralysis Virus, Chronic Bee Paralysis Virus and Deformed Wing Virus in Honey Bees: An Innovative and Non-Invasive Approach.Veterinary medicine and science · 2025Article
- Article
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Authors and funding
4 authors.
Funding
Abstract
Verifying the inclusivity of molecular detection methods gives indications about the reliability of viral infection diagnosis because of the tendency of viral pathogens to undergo sequence variation. This study was aimed at selecting inclusive probes based on reverse transcription-quantitative PCR (RT-qPCR) assays for the diagnosis of the most widespread and detrimental viruses infecting honeybees, namely the acute bee paralysis virus (ABPV), the black queen cell virus (BQCV), the chronic paralysis bee virus (CBPV), the deformed wing virus variants A (DWVA) and B (DWVB), and the sacbrood virus (SBV). Therefore, previously described detection methods were re-evaluated in silico for their specificity and inclusivity. Based on this evaluation, selected methods were modified, or new ones were designed and tested in duplex RT-qPCR reactions. The limits of detection (LODs), effect of multiplexing on sensitivity and the viral RNA quantification potential in bees and hive debris were assessed. This study made available diagnostic assays able to detect an increased number of virus variants compared with previously described tests and two viral pathogens in a single PCR reaction.
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Registered trials
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