Evidence map›Paper›PMID 38928414›Full record

ArticleInternational journal of molecular sciences2024

Changes in Telomere Length in Leukocytes and Leukemic Cells after Ultrashort Electron Beam Radiation.

Tigran Harutyunyan, Anzhela Sargsyan, Lily Kalashyan, Hovhannes Igityan, Bagrat Grigoryan, Hakob Davtyan, Rouben Aroutiounian, Thomas Liehr, Galina Hovhannisyan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Tigran HarutyunyanLaboratory of General and Molecular Genetics, Research Institute of Biology, Yerevan State University, Alex Manoogian 1, Yerevan 0025, Armenia.ORCID 0000-0002-1613-1019
Anzhela SargsyanLaboratory of General and Molecular Genetics, Research Institute of Biology, Yerevan State University, Alex Manoogian 1, Yerevan 0025, Armenia.ORCID 0000-0003-2720-0355
Lily KalashyanLaboratory of General and Molecular Genetics, Research Institute of Biology, Yerevan State University, Alex Manoogian 1, Yerevan 0025, Armenia.ORCID 0009-0004-0882-4984
Hovhannes IgityanLaboratory of General and Molecular Genetics, Research Institute of Biology, Yerevan State University, Alex Manoogian 1, Yerevan 0025, Armenia.
Bagrat GrigoryanCANDLE Synchrotron Research Institute, Acharyan 31, Yerevan 0040, Armenia.ORCID 0000-0003-4749-440X
Hakob DavtyanCANDLE Synchrotron Research Institute, Acharyan 31, Yerevan 0040, Armenia.ORCID 0000-0002-1046-1607
Rouben AroutiounianLaboratory of General and Molecular Genetics, Research Institute of Biology, Yerevan State University, Alex Manoogian 1, Yerevan 0025, Armenia.ORCID 0000-0003-1020-9513
Thomas LiehrInstitute of Human Genetics, Jena University Hospital, Friedrich Schiller University, Am Klinikum 1, D-07747 Jena, Germany.ORCID 0000-0003-1672-3054
Galina HovhannisyanLaboratory of General and Molecular Genetics, Research Institute of Biology, Yerevan State University, Alex Manoogian 1, Yerevan 0025, Armenia.ORCID 0000-0002-7450-1508

Funding

German Aerospace Center (= Deutsches Forschungszentrum für Luft- und Raumfahrt, DLR) 01DK24003RA MESCS Science Committee 21AG-1F068RA MESCS Science Committee and German Federal Ministry of Education and Research 22SC-BMBF-1C001
6 · The paper itself

Abstract

Application of laser-generated electron beams in radiotherapy is a recent development. Accordingly, mechanisms of biological response to radiation damage need to be investigated. In this study, telomere length (TL) as endpoint of genetic damage was analyzed in human blood cells (leukocytes) and K562 leukemic cells irradiated with laser-generated ultrashort electron beam. Metaphases and interphases were analyzed in quantitative fluorescence in situ hybridization (Q-FISH) to assess TL. TLs were shortened compared to non-irradiated controls in both settings (metaphase and interphase) after irradiation with 0.5, 1.5, and 3.0 Gy in blood leukocytes. Radiation also caused a significant TL shortening detectable in the interphase of K562 cells. Overall, a negative correlation between TL and radiation doses was observed in normal and leukemic cells in a dose-dependent manner. K562 cells were more sensitive than normal blood cells to increasing doses of ultrashort electron beam radiation. As telomere shortening leads to genome instability and cell death, the results obtained confirm the suitability of this biomarker for assessing genotoxic effects of accelerated electrons for their further use in radiation therapy. Observed differences in TL shortening between normal and K562 cells provide an opportunity for further development of optimal radiation parameters to reduce side effects in normal cells during radiotherapy.

Indexed as

ElectronsLeukocytesTelomereDNA DamageDose-Response Relationship, RadiationHumansIn Situ Hybridization, FluorescenceK562 CellsLeukemiaTelomere HomeostasisTelomere Shorteninghuman blood leukocytesionizing radiationK562 cell linelaser-generated electron beamsQ-FISHtelomere length

Identifiers

PMID38928414
PMCPMC11203595

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.