Evidence map›Paper›PMID 38926280›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Expression of Bispecific Antibodies Using Cellular Display.

Timothée Brunstein-Laplace, Maria Tsachaki, Megane Peltret, Roberto Giovannini, Martin Bertschinger

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Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Timothée Brunstein-LaplaceIchnos Sciences SA, La Chaux-de-Fonds, Switzerland.
Maria TsachakiIchnos Sciences SA, La Chaux-de-Fonds, Switzerland.
Megane PeltretIchnos Sciences SA, La Chaux-de-Fonds, Switzerland.
Roberto GiovanniniIchnos Sciences SA, La Chaux-de-Fonds, Switzerland.
Martin BertschingerIchnos Sciences SA, La Chaux-de-Fonds, Switzerland. Martin.Bertschinger@Ichnossciences.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The identification and selection of high-producing cell lines can be a resource- and time-consuming process. The screening effort can be simplified by assessing the potential for high expression (or a desired product quality attribute) of the individual cell directly in a mix of cells. Here, we describe protocols for the use of such a cellular display technology. Using alternate splicing, two mRNA constructs are generated at tunable ratios. The first mRNA codes for the secreted product, the second mRNA attaches a transmembrane domain to the antibody and directs it to the cellular membrane. The design of the basic construct as well as efficient ways to tune the strength of the cellular display is detailed in this chapter. Further, enrichment methods are provided enabling the flow cytometric sorting of a cell population based on the quantity of cellular display or on the product quality (heterodimerization level of a bispecific antibody).

Indexed as

Antibodies, BispecificFlow CytometryAlternative SplicingAnimalsHumansRNA, MessengerAntibodies, BispecificRNA, MessengerBispecific antibodyCellular displayFlow cytometryMultispecific antibody

Identifiers

PMID38926280

What OpenQuestion holds

Textmetadata
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.