Evidence map›Paper›PMID 38926275›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Recombinant Protein Production from Stable CHO Cell Pools.

Laurence Delafosse, Simon Lord-Dufour, Alex Pelletier, Sylvie Perret, Alina Burlacu, Manon Ouimet, Brian Cass, Simon Joubert, Matthew Stuible, Yves Durocher

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Laurence DelafosseHuman Health Therapeutics Research Centre, National Research Council Canada, Montreal, QC, Canada.
Simon Lord-DufourHuman Health Therapeutics Research Centre, National Research Council Canada, Montreal, QC, Canada.
Alex PelletierHuman Health Therapeutics Research Centre, National Research Council Canada, Montreal, QC, Canada.
Sylvie PerretHuman Health Therapeutics Research Centre, National Research Council Canada, Montreal, QC, Canada.
Alina BurlacuHuman Health Therapeutics Research Centre, National Research Council Canada, Montreal, QC, Canada.
Manon OuimetHuman Health Therapeutics Research Centre, National Research Council Canada, Montreal, QC, Canada.
Brian CassHuman Health Therapeutics Research Centre, National Research Council Canada, Montreal, QC, Canada.
Simon JoubertHuman Health Therapeutics Research Centre, National Research Council Canada, Montreal, QC, Canada.
Matthew StuibleHuman Health Therapeutics Research Centre, National Research Council Canada, Montreal, QC, Canada.
Yves DurocherHuman Health Therapeutics Research Centre, National Research Council Canada, Montreal, QC, Canada. Yves.durocher@nrc.gc.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The continuous improvement of expression platforms is necessary to respond to the increasing demand for recombinant proteins that are required to carry out structural or functional studies as well as for their characterization as biotherapeutics. While transient gene expression (TGE) in mammalian cells constitutes a rapid and well-established approach, non-clonal stably transfected cells, or "pools," represent another option, which is especially attractive when recurring productions of the same protein are required. From a culture volume of just a few liters, stable pools can provide hundreds of milligrams to gram quantities of high-quality secreted recombinant proteins.In this chapter, we describe a highly efficient and cost-effective procedure for the generation of Chinese Hamster Ovary cell stable pools expressing secreted recombinant proteins using commercially available serum-free media and polyethylenimine (PEI) as the transfection reagent. As a specific example of how this protocol can be applied, the production and downstream purification of recombinant His-tagged trimeric SARS-CoV-2 spike protein ectodomain (SmT1) are described.

Indexed as

CricetulusPolyethyleneimineRecombinant ProteinsSpike Glycoprotein, CoronavirusTransfectionAnimalsCHO CellsCricetinaeCulture Media, Serum-FreeSARS-CoV-2Culture Media, Serum-FreePolyethyleneimineRecombinant ProteinsSpike Glycoprotein, Coronavirusspike protein, SARS-CoV-2CHOHis-taggedIMACPEIPolyethylenimineRecombinant proteinSARS-CoV-2 spikeSerum-free mediumStable poolTransfection

Identifiers

PMID38926275

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.