Evidence map›Paper›PMID 38926271›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

The Use of Baculovirus-Mediated Gene Expression in Mammalian Cells for Recombinant Protein Production.

Julia Flock, Yexin Xie, Régis Lemaitre, Karine Lapouge, Kim Remans

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Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Julia FlockProtein Expression and Purification Core Facility, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
Yexin XieProtein Expression and Purification Core Facility, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
Régis LemaitreProtein Biochemistry Facility, Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), Dresden, Germany.
Karine LapougeProtein Expression and Purification Core Facility, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
Kim RemansProtein Expression and Purification Core Facility, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany. kim.remans@embl.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Baculovirus-mediated gene expression in mammalian cells, BacMam, is a useful alternative to transient transfection for recombinant protein production in various types of mammalian cell lines. We decided to establish BacMam in our lab in order to streamline our workflows for gene expression in insect and mammalian cells, as it is straightforward to parallelize the baculovirus generation for both types of eukaryotic cells. This chapter provides a step-by-step description of the protocols we use for the generation of the recombinant BacMam viruses, the transduction of mammalian cell cultures, and optimization of the protein production conditions through small-scale expression and purification tests.

Indexed as

BaculoviridaeGene ExpressionRecombinant ProteinsAnimalsCell Culture TechniquesCell LineGenetic VectorsHumansSf9 CellsTransduction, GeneticTransfectionRecombinant ProteinsBacMamBaculovirusCell cultureInsect cellsMammalian cellsProtein expressionProtein purification

Identifiers

PMID38926271

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.