Evidence map›Paper›PMID 38925526›Full record

ArticleNew biotechnology2024

Nucleic acid aptamers protect against lead (Pb(II)) toxicity.

Afreen Anwar, Solimar Ramis De Ayreflor Reyes, Aijaz Ahmad John, Erik Breiling, Abigail M O'Connor, Stephanie Reis, Jae-Hyuck Shim, Ali Asghar Shah, Jagan Srinivasan, Natalie G Farny

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in New biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Afreen AnwarDepartment of Biology and Biotechnology, Worcester Polytechnic Institute, 100 Institute Road, Worcester, MA 01609, USA; Department of Biotechnology, Baba Ghulam Shah Badshah University, Rajouri, J&K, India.
Solimar Ramis De Ayreflor ReyesDepartment of Biology and Biotechnology, Worcester Polytechnic Institute, 100 Institute Road, Worcester, MA 01609, USA.
Aijaz Ahmad JohnDepartment of Medicine, University of Massachusetts Chan Medical School, Worcester, MA, USA.
Erik BreilingDepartment of Biology and Biotechnology, Worcester Polytechnic Institute, 100 Institute Road, Worcester, MA 01609, USA.
Abigail M O'ConnorDepartment of Biology and Biotechnology, Worcester Polytechnic Institute, 100 Institute Road, Worcester, MA 01609, USA.
Stephanie ReisDepartment of Biology and Biotechnology, Worcester Polytechnic Institute, 100 Institute Road, Worcester, MA 01609, USA.
Jae-Hyuck ShimDepartment of Medicine, University of Massachusetts Chan Medical School, Worcester, MA, USA; Horae Gene Therapy Center, University of Massachusetts Chan Medical School, Worcester, MA, USA; Li Weibo Institute for Rare Diseases Research, University of Massachusetts Chan Medical School, Worcester, MA, USA.
Ali Asghar ShahDepartment of Biotechnology, Baba Ghulam Shah Badshah University, Rajouri, J&K, India.
Jagan SrinivasanDepartment of Biology and Biotechnology, Worcester Polytechnic Institute, 100 Institute Road, Worcester, MA 01609, USA; Program in Bioinformatics and Computational Biology, Worcester Polytechnic Institute, 100 Institute Road, Worcester, MA 01609, USA; Program in Neuroscience, Worcester Polytechnic Institute, 100 Institute Road, Worcester, MA 01609, USA.
Natalie G FarnyDepartment of Biology and Biotechnology, Worcester Polytechnic Institute, 100 Institute Road, Worcester, MA 01609, USA; Program in Bioinformatics and Computational Biology, Worcester Polytechnic Institute, 100 Institute Road, Worcester, MA 01609, USA; Program in Neuroscience, Worcester Polytechnic Institute, 100 Institute Road, Worcester, MA 01609, USA. Electronic address: nfarny@wpi.edu.

Funding

Identification of novel regulators governing osteoclast-osteoblast couplingR01AR068983 · NIAMS · WEILL MEDICAL COLL OF CORNELL UNIV · PI SHIM, JAE-HYUCK · 2016 to 2020
$1.9M
Novel approach to promote bone formation in osteoporosis using bone-homing mesenchymal stem cellsR21AR073331 · NIAMS · WEILL MEDICAL COLL OF CORNELL UNIV · PI SHIM, JAE-HYUCK, TUNG, CHING-HSUAN · 2018 to 2019
$425k
NIAMS NIH HHS R01 AR068983NIAMS NIH HHS R21 AR073331
6 · The paper itself

Abstract

Lead (Pb(II)) is a pervasive heavy metal toxin with many well-established negative effects on human health. Lead toxicity arises from cumulative, repeated environmental exposures. Thus, prophylactic strategies to protect against the bioaccumulation of lead could reduce lead-associated human pathologies. Here we show that DNA and RNA aptamers protect C. elegans from toxic phenotypes caused by lead. Reproductive toxicity, as measured by brood size assays, is prevented by co-feeding of animals with DNA or RNA aptamers. Similarly, lead-induced neurotoxicity, measured by behavioral assays, are also normalized by aptamer feeding. Further, cultured human HEK293 and primary murine osteoblasts are protected from lead toxicity by transfection with DNA aptamers. The osteogenic development, which is decreased by lead exposure, is maintained by prior transfection of lead-binding DNA aptamers. Aptamers may be an effective strategy for the protection of human health in the face of increasing environmental toxicants.

Indexed as

Aptamers, NucleotideCaenorhabditis elegansLeadAnimalsHEK293 CellsHumansMiceAptamers, NucleotideLeadAptamerBehaviorC. elegansLeadNeurotoxicityOsteoblastPb(II)Reproductive toxicity

Identifiers

PMID38925526
PMCPMC12987383

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.