ArticleThe Plant cell2024
The U-box E3 ubiquitin ligase PUB35 negatively regulates ABA signaling through AFP1-mediated degradation of ABI5.
Article in The Plant cell, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- The EAR motif-containing adaptor protein ECAP enhances ABI5-mediated ABA signaling to inhibit seed germination in Arabidopsis.Plant communications · 2026Article
- The trade-off mechanism between fiber elongation and seed vigor formation mediated by the RCD1-MYC3 module in cotton.Science China. Life sciences · 2026Article
- AHG1-AFP interaction as a regulatory node in the ABA response during seed germination.The Plant journal : for cell and molecular biology · 2026Article
- Lysine acetylome analysis reveals the critical role of acetylation-modified transcription factors and a chaperone protein in regulation of salt tolerance in Tamarix hispida.BMC plant biology · 2026Article
- SnRK2.6-mediated phosphorylation stabilizes proteasome regulator 1 (PTRE1) to enhance 26S proteasome activity and coordinate ABA signaling in Arabidopsis.Plant communications · 2026Article
- Genome-wide association analysis identifies novel genetic loci and candidate genes associated with seed dormancy in cultivated peanut.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Holistic overview of regulatory networks governing seed dormancy and germination in plants.Science China. Life sciences · 2026Review
- Genome-wide identification of the ATL gene family and their expression analysis in sweetpotato and its two diploid relatives.BMC plant biology · 2026Article
- OsDi19-1 Acts Downstream of OsABI5 to Negatively Regulate ABA-Inhibited Seed Germination and Seedling Growth in Rice.Rice (New York, N.Y.) · 2026Article
- Identification and characterization of U-box E3 ubiquitin ligase genes (ChPUBs) in Cardamine hupingshanensis under abiotic stress.BMC genomics · 2026Article
- Stage-specific regulation of seed germination under saline-alkali stress and implications for sequential emergence capacity.Frontiers in plant science · 2026Review
- Beyond light signaling: the broad regulatory spectrum of AFPs in plant growth and development.Frontiers in plant science · 2026Review
- Comprehensive Analysis of thePlants (Basel, Switzerland) · 2025Article
- Integrating Drought Stress Signaling and Smart Breeding for Climate-Resilient Crops: Regulatory Mechanisms and Genetic Strategies.Plants (Basel, Switzerland) · 2025Review
- OoAREB1 from Oxytropis ochrocephala binds to the promoters of stress-responsive genes to enhance their expression and improve drought tolerance.BMC plant biology · 2025Article
- A 14-3-3 modulator of seed weight and quality for unlocking the yield potential of soybean.Nature communications · 2025Article
- ODR1/AtSDR4L alleviates osmotic stress-induced germination arrest via ABA-dependent negative feedback on ABA biosynthesis.BMC plant biology · 2025Article
- Toward immortality: Natural variation in maize PROTEIN L-ISOASPARTYL O-METHYLTRANSFERASE 1 regulatory region shapes seed vigor and longevity.The Plant cell · 2025Article
- Precision tuning of ABA signaling by ubiquitination of ABA receptors: modulating protein activity and localization.Journal of experimental botany · 2025Article
- Genome-Wide Identification, Characterization, and Expression Analysis of the U-Box Gene Family in Cucumber (Plants (Basel, Switzerland) · 2025Article
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14 authors.
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Abstract
Abscisic acid (ABA) signaling is crucial for plant responses to various abiotic stresses. The Arabidopsis (Arabidopsis thaliana) transcription factor ABA INSENSITIVE 5 (ABI5) is a central regulator of ABA signaling. ABI5 BINDING PROTEIN 1 (AFP1) interacts with ABI5 and facilitates its 26S-proteasome-mediated degradation, although the detailed mechanism has remained unclear. Here, we report that an ABA-responsive U-box E3 ubiquitin ligase, PLANT U-BOX 35 (PUB35), physically interacts with AFP1 and ABI5. PUB35 directly ubiquitinated ABI5 in a bacterially reconstituted ubiquitination system and promoted ABI5 protein degradation in vivo. ABI5 degradation was enhanced by AFP1 in response to ABA treatment. Phosphorylation of the T201 and T206 residues in ABI5 disrupted the ABI5-AFP1 interaction and affected the ABI5-PUB35 interaction and PUB35-mediated degradation of ABI5 in vivo. Genetic analysis of seed germination and seedling growth showed that pub35 mutants were hypersensitive to ABA as well as to salinity and osmotic stresses, whereas PUB35 overexpression lines were hyposensitive. Moreover, abi5 was epistatic to pub35, whereas the pub35-2 afp1-1 double mutant showed a similar ABA response to the two single mutants. Together, our results reveal a PUB35-AFP1 module involved in fine-tuning ABA signaling through ubiquitination and 26S-proteasome-mediated degradation of ABI5 during seed germination and seedling growth.
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