Evidence map›Paper›PMID 38924022›Full record

ArticlePloS one2024

Spilanthes filicaulis (Schumach. & Thonn.) C. D Adam leaf extract prevents assault of streptozotocin on liver cells via inhibition of oxidative stress and activation of the NrF2/Keap1, PPARγ, and PTP1B signaling pathways.

Oluwafemi Adeleke Ojo, Fiyinfoluwa Stephen Oladepo, Akingbolabo Daniel Ogunlakin, Damilare IyinKristi Ayokunle, Adeshina Isaiah Odugbemi, Dare Ezekiel Babatunde, Adebola Busola Ojo, Omolola Adenike Ajayi-Odoko, Basiru Olaitan Ajiboye, Samuel Olatunde Dahunsi

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Article in PloS one, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

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No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Oluwafemi Adeleke OjoPhytomedicine, Molecular Toxicology and Computational Biochemistry Research Laboratory (PMTCB-RL), Biochemistry Programme, Bowen University, Iwo, Nigeria.ORCID 0000-0001-9331-396X
Fiyinfoluwa Stephen OladepoPhytomedicine, Molecular Toxicology and Computational Biochemistry Research Laboratory (PMTCB-RL), Biochemistry Programme, Bowen University, Iwo, Nigeria.
Akingbolabo Daniel OgunlakinPhytomedicine, Molecular Toxicology and Computational Biochemistry Research Laboratory (PMTCB-RL), Biochemistry Programme, Bowen University, Iwo, Nigeria.ORCID 0000-0002-1649-846X
Damilare IyinKristi AyokunlePure and Applied Biology Programme, Bowen University, Iwo, Nigeria.
Adeshina Isaiah OdugbemiPhytomedicine, Molecular Toxicology and Computational Biochemistry Research Laboratory (PMTCB-RL), Biochemistry Programme, Bowen University, Iwo, Nigeria.
Dare Ezekiel BabatundeAnatomy Programme, Bowen University, Iwo, Nigeria.
Adebola Busola OjoDepartment of Biochemistry, Ekiti State University, Ado-Ekiti, Nigeria.
Omolola Adenike Ajayi-OdokoMicrobiology Programme, Bowen University, Iwo, Nigeria.
Basiru Olaitan AjiboyeDepartment of Biochemistry, Federal University, Oye-Ekiti, Oye-Ekiti, Nigeria.
Samuel Olatunde DahunsiMicrobiology Programme, Bowen University, Iwo, Nigeria.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundSpilanthes filicaulis (Schumach. & Thonn.) C. D Adam is a shrubby plant of the Asteraceae family that has medicinal benefits for the pharmaceutical and cosmetic industries. PURPOSE: The purpose of this study was to assess the effectiveness of Spilanthes filicaulis leaf extract in a streptozotocin (STZ)-induced rat model and the associated signaling pathways.

methodsA sample of 25 male Wistar rats was randomly assigned to groups I, II, III, IV, and V. Each group included five animals, i.e., control rats, diabetic control rats, diabetic rats treated with metformin, and diabetic rats treated with 150 mg/kg/bw and 300 mg/kg/bw of the methanolic extract of S. filicaulis leaves (MESFL). Treatment was administered for 15 successive days via oral gavage. After 15 days, the rats were evaluated for fasting blood glucose (FBG), glycated hemoglobin (HbA1c), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), triglyceride (TG), total cholesterol (TC), low-density lipoprotein (LDL), high-density lipoprotein (HDL), reduced glutathione (GSH), glutathione-S-transferase (GST), superoxide dismutase (SOD), catalase (CAT), lipid peroxidation (MDA), hexokinase, and glucose-6-phosphatase activities. Gene expression levels of nuclear factor erythroid 2-related factor 2 (Nrf2), peroxisome proliferator-activated receptor gamma (PPAR-γ), kelch-like ECH-associated protein 1 (Keap1), protein tyrosine phosphatase 1B (PTP1B) and the antiapoptotic protein caspase-3 were examined.

resultsMESFL was administered to diabetic rats, and changes in body weight, fasting blood glucose (FBG) and HbA1c were restored. Furthermore, in diabetic rats, S. filicaulis significantly reduced the levels of triglycerides (TGs), total cholesterol (TC), low-density lipoprotein (LDL), and very low-density lipoprotein (VLDL) and significantly increased HDL. S. filicaulis improved ALT, AST, and ALP enzyme activity in diabetic rats. MDA levels decreased considerably with increasing activity of antioxidant enzymes, such as GST, SOD, CAT and GSH, in diabetic liver rats treated with S. filicaulis. Diabetic rats treated with MESFL and metformin exhibited upregulated mRNA expression levels of nuclear factor erythroid 2-related factor 2 (Nrf2) and peroxisome proliferator-activated receptor gamma (PPAR-γ). Kelch-like ECH-associated protein 1 (Keap1) and protein tyrosine phosphatase 1B (PTP1B) mRNA expression in the liver was downregulated in diabetic rats treated with MESFL and metformin. In addition, MESFL downregulated the mRNA expression of caspase-3 in diabetic rats.

conclusionIt can be concluded from the data presented in this study that MESFL exerts a protective effect on diabetic rats due to its antidiabetic, antioxidant, antihyperlipidemic and antiapoptotic effects and may be considered a treatment for T2DM.

Indexed as

Diabetes Mellitus, ExperimentalKelch-Like ECH-Associated Protein 1LiverNF-E2-Related Factor 2Oxidative StressPlant ExtractsPlant LeavesPPAR gammaProtein Tyrosine Phosphatase, Non-Receptor Type 1Rats, WistarSignal TransductionAnimalsAsteraceaeHypoglycemic AgentsMaleRatsHypoglycemic AgentsKEAP1 protein, ratKelch-Like ECH-Associated Protein 1Nfe2l2 protein, ratNF-E2-Related Factor 2Plant ExtractsPPAR gammaPPAR gamma, ratProtein Tyrosine Phosphatase, Non-Receptor Type 1Ptpn1 protein, ratStreptozocin

Identifiers

PMID38924022
PMCPMC11207034

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.