ArticleThe Journal of general virology2024
H5N1 clade 2.3.4.4b avian influenza viruses replicate in differentiated bovine airway epithelial cells cultured at air-liquid interface.
Article in The Journal of general virology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Beyond H5N1: influenza A virus infection in bovine udder organoids.Emerging microbes & infections · 2026Article
- Airway organoids reveal patterns of influenza A tropism and adaptation in wildlife species.Emerging microbes & infections · 2026Article
- Animal organoids as models for integrated One Health research.One health (Amsterdam, Netherlands) · 2026Review
- Cellular tropism of highly pathogenic avian influenza (HPAI) A(H5N1) clade 2.3.4.4b viruses in human respiratory and bovine mammary epithelial cells.Microbiology spectrum · 2026Article
- IFNL3 reduces replication of CIRDC-associated viruses in canine airway epithelial cells.Virus research · 2026Article
- Detection of antibodies against avian influenza in European dairy cattle, the Netherlands, January 2026.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2026Article
- Animal organoids as transformative platforms for viral infections and zoonotic cross-species viral research.Journal of virology · 2026Review
- Efficient replication of avian, porcine and human influenza A viruses in well-differentiated bovine airway epithelial cells.Emerging microbes & infections · 2025Article
- Susceptibility of calf lung slice cultures to H5N1 influenza virus.Emerging microbes & infections · 2025Article
- Risk of infection of dairy cattle in the EU with highly pathogenic avian influenza virus affecting dairy cows in the United States of America (H5N1, Eurasian lineage goose/Guangdong clade 2.3.4.4b. genotype B3.13).EFSA journal. European Food Safety Authority · 2025Article
- Ocular infectivity and replication of a clade 2.3.4.4b A(H5N1) influenza virus associated with human conjunctivitis in a dairy farm worker in the USA: an in-vitro and ferret study.The Lancet. Microbe · 2025Article
- Transmission and pathogenicity in ferrets after experimental infection with HPAI clade 2.3.4.4b H5N1 viruses.The Journal of general virology · 2025Article
- Experimental infection of chickens, Pekin ducks, Eurasian wigeons and Barnacle geese with two recent highly pathogenic avian influenza H5N1 clade 2.3.4.4b viruses.Emerging microbes & infections · 2024Article
- Article
- Avian influenza overview March-June 2024.EFSA journal. European Food Safety Authority · 2024Article
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Highly pathogenic avian influenza (HPAI) H5N1 viruses are responsible for disease outbreaks in wild birds and poultry, resulting in devastating losses to the poultry sector. Since 2020, an increasing number of outbreaks of HPAI H5N1 was seen in wild birds. Infections in mammals have become more common, in most cases in carnivores after direct contact with infected birds. Although ruminants were previously not considered a host species for HPAI viruses, in March 2024 multiple outbreaks of HPAI H5N1 were detected in goats and cattle in the United States. Here, we have used primary bronchus-derived well-differentiated bovine airway epithelial cells (WD-AECs) cultured at air-liquid interface to assess the susceptibility and permissiveness of bovine epithelial cells to infection with European H5N1 virus isolates. We inoculated bovine WD-AECs with three low-passage HPAI clade 2.3.4.4b H5N1 virus isolates and detected rapid increases in viral genome loads and infectious virus during the first 24 h post-inoculation, without substantial cytopathogenic effects. Three days post-inoculation infected cells were still detectable by immunofluorescent staining. These data indicate that multiple lineages of HPAI H5N1 may have the propensity to infect the respiratory tract of cattle and support extension of avian influenza surveillance efforts to ruminants. Furthermore, this study underscores the benefit of WD-AEC cultures for pandemic preparedness by providing a rapid and animal-free assessment of the host range of an emerging pathogen.
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