Evidence map›Paper›PMID 38922499›Full record

ArticleGenes & genomics2024

Transcriptome analysis of East Asian common octopus, Octopus sinensis, paralarvae.

Ki Tae Kim, Mi Ae Kim, Woo Jin Kim, Min Min Jung, Dong Hwi Kim, Young Chang Sohn

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Article in Genes & genomics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Ki Tae KimSoutheast Sea Fisheries Research Institute, National Institute of Fisheries Science, Tongyeong, Gyeongsangnam-Do, 53017, Republic of Korea.
Mi Ae KimDepartment of Marine Bioscience, Gangneung-Wonju National University, Gangneung, Gangwon-Do, 25457, Republic of Korea.
Woo Jin KimBiotechnology Research Division, National Institute of Fisheries Science, Busan, 46083, Republic of Korea.
Min Min JungSubtropical Fisheries Research Institute, National Institute of Fisheries Science, Jeju-do, 63068, Republic of Korea.
Dong Hwi KimEast Sea Fisheries Research Institute, National Institute of Fisheries Science, Gangneung, Gangwon-Do, 25435, Republic of Korea.
Young Chang SohnDepartment of Marine Bioscience, Gangneung-Wonju National University, Gangneung, Gangwon-Do, 25457, Republic of Korea. ycsohn@gwnu.ac.kr.ORCID http://orcid.org/0000-0002-4140-2669

Funding

Ministry of Oceans and Fisheries R2024047
6 · The paper itself

Abstract

backgroundThe genes involved in cephalopod development and their association with hatching and survival during early life stages have been extensively studied. However, few studies have investigated the paralarvae transcriptome of the East Asian common octopus (Octopus sinen sis).

objectiveThis study aimed to identify the genes related to embryonic development and hatching in O. sinensis using RNA sequencing (RNA-seq) and verify the genes most relevant to different embryonic stages.

methodsRNA samples from hatched and 25 days post-hatching (dph) O. sinensis paralarvae were used to construct cDNA libraries. Clean reads from individual samples were aligned to the reference O. sinensis database to identify the differentially expressed genes (DEGs) between the 0- and 25-dph paralarvae libraries. Real-time quantitative polymerase chain reaction (RT-qPCR) was used to supplement the RNA-seq data for embryogenic developmental stages.

resultsA total of 12,597 transcripts were annotated and 5,468 DEGs were identified between the 0- and 25-dph O. sinensis paralarvae, including 2,715 upregulated and 2,753 downregulated transcripts in the 25-dph paralarvae. Several key DEGs were related to transmembrane transport, lipid biosynthesis, monooxygenase activity, lipid transport, neuropeptide signaling, transcription regulation, and protein-cysteine S-palmitoyltransferase activity during the post-hatching development of O. sinensis paralarvae. RT-qPCR analysis further revealed that SLC5A3A, ABCC12, and NPC1 transcripts in 20 and/or 30 days post-fertilization (dpf) embryos were significantly higher (p < 0.05) than those in 10-dpf embryos.

conclusionTranscriptome profiles provide molecular targets to understand the embryonic development, hatching, and survival of O. sinensis paralarvae, and enhance octopus production.

Indexed as

OctopodiformesTranscriptomeAnimalsGene Expression ProfilingGene Expression Regulation, DevelopmentalCephalopodDEGEmbryoOctopus sinensisRNA sequencing

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.