ArticleBiosensors2024
Colorimetric Glucose Biosensor Based on Chitosan Films and Its Application for Glucose Detection in Beverages Using a Smartphone Application.
Article in Biosensors, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- A wearable closed-loop insulin micropump toward a miniaturized artificial pancreas for diabetes.Microsystems & nanoengineering · 2026Article
- Optical Biosensors-Principles of Operation and Applications.Micromachines · 2026Review
- Covalent immobilization of Lepidium draba peroxidase on chitosan-coated magnetic nanoparticles and its application in glucose biosensing.Scientific reports · 2026Article
- Paper-Based Device for the Colorimetric Determination of Glucose in Whole-Blood Samples Using a Smartphone.Bioengineering (Basel, Switzerland) · 2025Article
- Comparative Roles of Hydrogels, Deep Eutectic Solvents, and Ionic Liquids in Enzyme-Based Biosensors, Bioelectronics and Biomimetics Devices.ACS measurement science au · 2025Review
- 3D-Printed PLA Hollow Microneedles Loaded with Chitosan Nanoparticles for Colorimetric Glucose Detection in Sweat Using Machine Learning.Biosensors · 2025Article
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Authors and funding
4 authors.
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Abstract
Nowadays, biosensors are gaining increasing interest in foods' and beverages' quality control, owing to their economic production, enhanced sensitivity, specificity, and faster analysis. In particular, colorimetric biosensors can be combined with color recognition applications on smartphones for the detection of analytes, rendering the whole procedure more applicable in everyday life. Herein, chitosan (CS) films were prepared with the deep eutectic solvent (DES) choline chloride/urea/glycerol (ChCl:U:Gly). Glucose oxidase (GOx), a widely utilized enzyme in quality control, was immobilized within CS films through glutaraldehyde (GA), leading to the formation of CS/GOx films. The optimized GOx concentration and DES content were determined for the films. Moreover, the effect of the pH and temperature of the glucose oxidation reaction on the enzymatic activity of GOx was studied. The structure, stability, and specificity of the CS/GOx films as well as the Km values of free and immobilized GOx were also determined. Finally, the analytical performance of the films was studied by using both a spectrophotometer and a color recognition application on a smartphone. The results demonstrated that the films were highly accurate, specific to glucose, and stable when stored at 4 °C for 4 weeks and when reused 10 times, without evident activity loss. Furthermore, the films displayed a good linear response range (0.1-0.8 mM) and a good limit of detection (LOD, 33 μM), thus being appropriate for the estimation of glucose concentration in real samples through a smartphone application.
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