Evidence map›Paper›PMID 38918798›Full record

ArticleEvoDevo2024

See-Star: a versatile hydrogel-based protocol for clearing large, opaque and calcified marine invertebrates.

D N Clarke, L Formery, C J Lowe

Abstract read
In one paragraph

Article in EvoDevo, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

D N Clarke *Department of Biology, Hopkins Marine Station, Stanford University, Pacific Grove, CA, USA. clarken@mit.edu.ORCID http://orcid.org/0000-0002-9774-2582
L Formery *Department of Biology, Hopkins Marine Station, Stanford University, Pacific Grove, CA, USA.ORCID http://orcid.org/0000-0003-0230-4051
C J LoweDepartment of Biology, Hopkins Marine Station, Stanford University, Pacific Grove, CA, USA.ORCID http://orcid.org/0000-0002-7789-8643

Funding

National Science Foundation 1656628
6 · The paper itself

Abstract

Studies of morphology and developmental patterning in adult stages of many invertebrates are hindered by opaque structures, such as shells, skeletal elements, and pigment granules that block or refract light and necessitate sectioning for observation of internal features. An inherent challenge in studies relying on surgical approaches is that cutting tissue is semi-destructive, and delicate structures, such as axonal processes within neural networks, are computationally challenging to reconstruct once disrupted. To address this problem, we developed See-Star, a hydrogel-based tissue clearing protocol to render the bodies of opaque and calcified invertebrates optically transparent while preserving their anatomy in an unperturbed state, facilitating molecular labeling and observation of intact organ systems. The resulting protocol can clear large (> 1 cm

Indexed as

ClearingEchinodermsHydrogelMarine invertebratesMolluscs

Identifiers

PMID38918798
PMCPMC11201320

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.