ArticleNature communications2024
Overtone photothermal microscopy for high-resolution and high-sensitivity vibrational imaging.
Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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Who cites it
6 citing papers in PubMed.
- Stimulated Raman photothermal microscopy: theory and implementation.Advances in optics and photonics · 2026Article
- Far-field single-molecule vibrational spectroscopy and imaging.Chemical science · 2026Review
- Not Just Better Resolution: A Detailed Study of the Signal Distribution in Mid-Infrared Optical Photothermal Imaging.Analytical chemistry · 2025Article
- Ultrasensitive in vivo infrared spectroscopic imaging via oblique photothermal microscopy.Nature communications · 2025Article
- Advanced vibrational microscopes for life science.Nature methods · 2025Review
- Fiber laser based stimulated Raman photothermal microscopy towards a high-performance and user-friendly chemical imaging platform.PhotoniX · 2025Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Photothermal microscopy is a highly sensitive pump-probe method for mapping nanostructures and molecules through the detection of local thermal gradients. While visible photothermal microscopy and mid-infrared photothermal microscopy techniques have been developed, they possess inherent limitations. These techniques either lack chemical specificity or encounter significant light attenuation caused by water absorption. Here, we present an overtone photothermal (OPT) microscopy technique that offers high chemical specificity, detection sensitivity, and spatial resolution by employing a visible probe for local heat detection in the C-H overtone region. We demonstrate its capability for high-fidelity chemical imaging of polymer nanostructures, depth-resolved intracellular chemical mapping of cancer cells, and imaging of multicellular C. elegans organisms and highly scattering brain tissues. By bridging the gap between visible and mid-infrared photothermal microscopy, OPT establishes a new modality for high-resolution and high-sensitivity chemical imaging. This advancement complements large-scale shortwave infrared imaging approaches, facilitating multiscale structural and chemical investigations of materials and biological metabolism.
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Registered trials
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