ArticleNature communications2024
Raman micro-spectroscopy reveals the spatial distribution of fumarate in cells and tissues.
Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Itaconation and Succination as Metabolite-Derived Cysteine Modifications in Inflammatory Signalling: Biochemical Mechanisms, Chemoproteomic Mapping, and Therapeutic Opportunities.Cell biochemistry and biophysics · 2026Review
- All-optical multimodal mapping of single-cell-type-specific metabolic activities via REDCAT.Nature methods · 2026Article
- Label-Free Raman Spectroscopy Reveals Metabolic Signatures Associated with MGMT Promoter Methylation Status in Glioblastoma.Analytical chemistry · 2026Article
- Biochemical Signatures of L-Carnitine-Induced Changes in Brain Cancer Cells Revealed by Confocal Raman Imaging: A Preliminary Study.Sensors (Basel, Switzerland) · 2026Article
- SPEND-hSRS imaging of fumarate uncovers mitochondrial metabolic heterogeneity.bioRxiv : the preprint server for biology · 2026Article
- Open Raman Microscopy (ORM): A Modular Hardware and Software Framework for Accessible Raman Imaging.bioRxiv : the preprint server for biology · 2025Article
- Confocal Raman microspectroscopy for spatially resolved tissue characterisation of disease-linked spectra-pathological signatures.Clinical and translational medicine · 2025Review
- A high throughput strategy for traditional Chinese medicine active compound screening based on Raman spectroscopy.Journal of pharmaceutical analysis · 2025Article
- Innovative design and comprehensive characterization of a valine-enhanced lipid nanoparticle-coated mRNA vaccine targeting the SARS-CoV-2 S-RBD antigen.Scientific reports · 2025Article
- Advanced vibrational microscopes for life science.Nature methods · 2025Review
- Development and Testing of a Novel Microstrip Photocathode ICCD for Lunar Remote Raman Detection.Sensors (Basel, Switzerland) · 2025Article
- Machine learning-enhanced confocal Raman imaging enables label-free diagnosis and spatial metabolic profiling of isoniazid-induced hepatotoxicity.Theranostics · 2025Article
- Oncometabolites at the crossroads of genetic, epigenetic and ecological alterations in cancer.Cell death and differentiation · 2024Review
- Imaging immunometabolismImmunometabolism (Cobham, Surrey) · 2024Article
- Rewiring of RNA methylation by the oncometabolite fumarate in renal cell carcinoma.NAR cancer · 2024Article
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Authors and funding
10 authors.
Funding
Abstract
Aberrantly accumulated metabolites elicit intra- and inter-cellular pro-oncogenic cascades, yet current measurement methods require sample perturbation/disruption and lack spatio-temporal resolution, limiting our ability to fully characterize their function and distribution. Here, we show that Raman spectroscopy (RS) can directly detect fumarate in living cells in vivo and animal tissues ex vivo, and that RS can distinguish between Fumarate hydratase (Fh1)-deficient and Fh1-proficient cells based on fumarate concentration. Moreover, RS reveals the spatial compartmentalization of fumarate within cellular organelles in Fh1-deficient cells: consistent with disruptive methods, we observe the highest fumarate concentration (37 ± 19 mM) in mitochondria, where the TCA cycle operates, followed by the cytoplasm (24 ± 13 mM) and then the nucleus (9 ± 6 mM). Finally, we apply RS to tissues from an inducible mouse model of FH loss in the kidney, demonstrating RS can classify FH status. These results suggest RS could be adopted as a valuable tool for small molecule metabolic imaging, enabling in situ non-destructive evaluation of fumarate compartmentalization.
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