Evidence map›Paper›PMID 38918384›Full record

ArticleNature communications2024

MiDAS 5: Global diversity of bacteria and archaea in anaerobic digesters.

Morten Kam Dahl Dueholm, Kasper Skytte Andersen, Anne-Kirstine C Korntved, Vibeke Rudkjøbing, Madalena Alves, Yadira Bajón-Fernández, Damien Batstone, Caitlyn Butler, Mercedes Cecilia Cruz, Åsa Davidsson and 19 more

Abstract read
In one paragraph

Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.

0numbers the graph read from it
0cells of the map it votes in
35citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

35 citing papers in PubMed.

  1. Review
  2. Article
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  6. Article
  7. Selective survival of anaerobic digester microbes afterWater science and technology : a journal of the International Association on Water Pollution Research · 2026
    Article
  8. Applied and environmental microbiology · 2026
    Article
  9. How to quantify immigration from community abundance data using the neutral community model.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

29 authors.

Morten Kam Dahl DueholmCenter for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University, Aalborg, Denmark. md@bio.aau.dk.ORCID http://orcid.org/0000-0003-4135-2670
Kasper Skytte AndersenCenter for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University, Aalborg, Denmark.ORCID http://orcid.org/0000-0002-5423-0958
Anne-Kirstine C KorntvedCenter for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University, Aalborg, Denmark.
Vibeke RudkjøbingCenter for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University, Aalborg, Denmark.
Madalena AlvesCentre of Biological Engineering, University of Minho, Minho, Portugal.
Yadira Bajón-FernándezSchool of Water, Energy and Environment, Cranfield University, Cranfield, UK.ORCID http://orcid.org/0000-0002-0939-235X
Damien BatstoneAustralian Centre for Water and Environmental Biotechnology (ACWEB), The University of Queensland, Brisbane, Australia.
Caitlyn ButlerDepartment of Civil and Environmental Engineering, University of Massachusetts Amherst, Amherst, MA, USA.
Mercedes Cecilia CruzInstituto de Investigaciones para la Industria Química (INIQUI), Universidad Nacional de Salta (UNSa) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Salta, Argentina.
Åsa DavidssonDepartment of Chemical Engineering, Lund University, Lund, Sweden.ORCID http://orcid.org/0000-0002-2981-6551
Leonardo ErijmanINGEBI-CONICET, University of Buenos Aires, Buenos Aires, Argentina.
Christof HolligerLaboratory for Environmental Biotechnology, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.ORCID http://orcid.org/0000-0002-9645-3834
Konrad KochChair of Urban Water Systems Engineering, Technical University of Munich (TUM), Garching, Germany.ORCID http://orcid.org/0000-0001-5425-0169
Norbert KreuzingerInstitute of Water Quality and Resource Management, TU Wien, Vienna, Austria.
Changsoo LeeDepartment of Civil, Urban, Earth, and Environmental Engineering & Graduate School of Carbon Neutrality, Ulsan National Institute of Science and Technology (UNIST), Ulsan, South Korea.
Gerasimos LyberatosSchool of Chemical Engineering, National Technical University of Athens, Zografou, Greece.ORCID http://orcid.org/0000-0003-1663-4035
Srikanth MutnuriApplied Environmental Biotechnology Laboratory, Birla Institute of Technology and Science (BITS-Pilani), Pilani, Goa campus, Goa, India.
Vincent O'FlahertySchool of Biological and Chemical Sciences and Ryan Institute, University of Galway, Galway, Ireland.
Piotr Oleskowicz-PopielWater Supply and Bioeconomy Division, Faculty of Environmental Engineering and Energy, Poznan University of Technology, Poznan, Poland.
Dana PokornaDepartment of Water Technology and Environmental Engineering, University of Chemistry and Technology Prague, Prague, Czech Republic.
Veronica RajalInstituto de Investigaciones para la Industria Química (INIQUI), Universidad Nacional de Salta (UNSa) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Salta, Argentina.
Michael RecktenwaldResearch Scientist at Kemira Oyj, Espoo R&D Center, Espoo, Finland.
Jorge RodríguezChemical Engineering Department, Khalifa University, Khalifa, UAE.ORCID http://orcid.org/0000-0002-5936-9676
Pascal E SaikalyEnvironmental Science and Engineering Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Kingdom of Saudi Arabia.ORCID http://orcid.org/0000-0001-7678-3986
Nick TookerDepartment of Civil and Environmental Engineering, University of Massachusetts Amherst, Amherst, MA, USA.
Julia VierheiligInstitute of Water Quality and Resource Management, TU Wien, Vienna, Austria.ORCID http://orcid.org/0000-0002-9764-7534
Jo De VriezeCenter for Microbial Ecology and Technology (CMET), Ghent University, Ghent, Belgium.ORCID http://orcid.org/0000-0001-9365-8896
Christian WurzbacherChair of Urban Water Systems Engineering, Technical University of Munich (TUM), Garching, Germany.
Per Halkjær NielsenCenter for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University, Aalborg, Denmark. phn@bio.aau.dk.ORCID http://orcid.org/0000-0002-6402-1877

Funding

Det Frie Forskningsråd (Danish Council for Independent Research) 6111-00617 AVillum Fonden (Villum Foundation) Dark Matter and grant 13351
6 · The paper itself

Abstract

Anaerobic digestion of organic waste into methane and carbon dioxide (biogas) is carried out by complex microbial communities. Here, we use full-length 16S rRNA gene sequencing of 285 full-scale anaerobic digesters (ADs) to expand our knowledge about diversity and function of the bacteria and archaea in ADs worldwide. The sequences are processed into full-length 16S rRNA amplicon sequence variants (FL-ASVs) and are used to expand the MiDAS 4 database for bacteria and archaea in wastewater treatment systems, creating MiDAS 5. The expansion of the MiDAS database increases the coverage for bacteria and archaea in ADs worldwide, leading to improved genus- and species-level classification. Using MiDAS 5, we carry out an amplicon-based, global-scale microbial community profiling of the sampled ADs using three common sets of primers targeting different regions of the 16S rRNA gene in bacteria and/or archaea. We reveal how environmental conditions and biogeography shape the AD microbiota. We also identify core and conditionally rare or abundant taxa, encompassing 692 genera and 1013 species. These represent 84-99% and 18-61% of the accumulated read abundance, respectively, across samples depending on the amplicon primers used. Finally, we examine the global diversity of functional groups with known importance for the anaerobic digestion process.

Indexed as

ArchaeaBacteriaBiodiversityMicrobiotaPhylogenyRNA, Ribosomal, 16SAnaerobiosisBioreactorsMethaneSequence Analysis, DNAWastewaterMethaneRNA, Ribosomal, 16SWastewater

Identifiers

PMID38918384
PMCPMC11199495

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.