ArticleInternational dental journal2025
In Vitro Effect of Streptococcus mutans Biofilm Produced in Sugar-Free Coca-Cola on Enamel.
Article in International dental journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Early Childhood Caries and Oral Hygiene Practices in Preschool Children in Dubai: A Cross-sectional Study.International journal of clinical pediatric dentistry · 2026Article
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Authors and funding
6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
objectiveSugary drinks such as Coca-Cola may expedite dental caries. For this reason, sugar-free drinks like Coca-Cola Zero Sugar (CZ) may be considered advantageous. This research aims to evaluate in vitro the CZ effect in the presence of Streptococcus mutans (S. mutans) biofilm on enamel demineralization.
methodsNinety-six human enamel slabs (4 × 4 mm) were used. S. mutans UA-159 72-hour biofilm was created over enamel surfaces. The specimens were soaked in CZ, HCl, or 10% sucrose in PBS solution, 3 times a day for 15 minutes over the course of 4 days. Viable counts (CFU/mL) and biofilm biomass (Crystal Violet staining) were evaluated. pH was measured after each exposure. After 4 days, Demineralization was evaluated clinically and by Vickers microhardness tests. Slabs were photographed using a stereomicroscope before and after exposure to caries-promoting conditions.
resultsSlabs that were soaked in CZ showed an increase in viable counts compared to control and almost similar counts with 10% sucrose in PBS solution exposures (10
conclusionsCZ creates a favourable environment for the growth of S. mutans. It may be suggested that even though CZ is sugar free it has a cariogenic effect on enamel. CLINICAL SIGNIFICANCE: Clinicians need to educate patients that sugar-free carbonated drinks may be just as harmful as regular carbonated drinks, and hence avoided. This research emphasizes the harmful effect sugar-free carbonated drinks on teeth and sheds new light on their cariogenic potential.
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