Evidence map›Paper›PMID 38917817›Full record

ArticleThe American journal of tropical medicine and hygiene2024

Using Dried Blood Spots to Quantitatively Detect Anti-SARS-CoV-2 IgG Antibodies by ELISA: A Validation Study.

Michelle Ylade, Asma Binte Aziz, Jedas Veronica Daag, Maria Vinna Crisostomo, Kristal-An Agrupis, Maria Angela Maronilla, Chloe Sye Lim Hong, Hwa Young Kim, Irene Njau, March Helena Jane Lopez and 6 more

Abstract readValidation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in The American journal of tropical medicine and hygiene, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Michelle YladeInstitute of Child Health and Human Development, National Institutes of Health, University of the Philippines-Manila, Manila, Philippines.
Asma Binte AzizInternational Vaccine Institute, Seoul, Korea.
Jedas Veronica DaagInstitute of Child Health and Human Development, National Institutes of Health, University of the Philippines-Manila, Manila, Philippines.
Maria Vinna CrisostomoInstitute of Child Health and Human Development, National Institutes of Health, University of the Philippines-Manila, Manila, Philippines.
Kristal-An AgrupisInstitute of Child Health and Human Development, National Institutes of Health, University of the Philippines-Manila, Manila, Philippines.
Maria Angela MaronillaInstitute of Child Health and Human Development, National Institutes of Health, University of the Philippines-Manila, Manila, Philippines.
Chloe Sye Lim HongInternational Vaccine Institute, Seoul, Korea.
Hwa Young KimInternational Vaccine Institute, Seoul, Korea.
Irene NjauInternational Vaccine Institute, Seoul, Korea.
March Helena Jane LopezInstitute of Child Health and Human Development, National Institutes of Health, University of the Philippines-Manila, Manila, Philippines.
Jacqueline DeenInstitute of Child Health and Human Development, National Institutes of Health, University of the Philippines-Manila, Manila, Philippines.
Deok Ryun KimInternational Vaccine Institute, Seoul, Korea.
Young Ae YouInternational Vaccine Institute, Seoul, Korea.
Sophie S Y KangInternational Vaccine Institute, Seoul, Korea.
Florian MarksInternational Vaccine Institute, Seoul, Korea.
Birkneh Tilahun TadesseInternational Vaccine Institute, Seoul, Korea.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

SARS-CoV-2 serological testing is useful to determine seroprevalence, epidemiological trends, and the extent of transmission. The collection and transport of serum samples can be logistically challenging, especially in remote underserved areas. Dried blood spots (DBSs) would allow easier sample collection and logistical handling compared with standard serum collection, particularly for extensive and repeated SARS-CoV-2 serosurveys. We evaluated the sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of the IgG ELISA (Wantai, Beijing, China) using DBSs against sera for the quantitative detection of SARS-CoV-2 IgG antibody. The IgG ELISA was used to test paired sera and DBSs obtained from individuals with recent virologically confirmed COVID-19 illness and banked paired sera and DBSs collected before the COVID-19 pandemic. We found that 100/100 (100%) seropositive samples were positive using DBSs, and 193/194 (99%) seronegative samples were negative using DBSs. Compared with sera, the DBS method had a 100% sensitivity, 99% specificity, 99% PPV, and 100% NPV. Use of DBSs for SARS-CoV-2 household or population serosurveys may be considered in situations with limitations in sample collection, shipment, and storage.

Indexed as

Antibodies, ViralCOVID-19COVID-19 Serological TestingEnzyme-Linked Immunosorbent AssayImmunoglobulin GSARS-CoV-2Sensitivity and SpecificityAdultDried Blood Spot TestingFemaleHumansMaleMiddle AgedSeroepidemiologic StudiesSpecimen HandlingAntibodies, ViralImmunoglobulin G

Identifiers

PMID38917817
PMCPMC11310613

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.