Evidence map›Paper›PMID 38915627›Full record

ArticlebioRxiv : the preprint server for biology2024

Enabling non-viral DNA delivery using lipid nanoparticles co-loaded with endogenous anti-inflammatory lipids.

Manthan N Patel, Sachchidanand Tiwari, Yufei Wang, Sarah O'Neill, Jichuan Wu, Serena Omo-Lamai, Carolann Espy, Liam S Chase, Aparajeeta Majumdar, Evan Hoffman and 5 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

15 authors.

Manthan N PatelDepartment of Systems Pharmacology and Translational Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.ORCID 0000-0002-0691-8424
Sachchidanand TiwariDivision of Pulmonary, Allergy, and Critical Care, Department of Medicine, Perelman School of Medicine University of Pennsylvania Philadelphia, PA, USA.
Yufei WangDivision of Pulmonary, Allergy, and Critical Care, Department of Medicine, Perelman School of Medicine University of Pennsylvania Philadelphia, PA, USA.
Sarah O'NeillDepartment of Systems Pharmacology and Translational Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Jichuan WuDivision of Pulmonary, Allergy, and Critical Care, Department of Medicine, Perelman School of Medicine University of Pennsylvania Philadelphia, PA, USA.
Serena Omo-LamaiDivision of Pulmonary, Allergy, and Critical Care, Department of Medicine, Perelman School of Medicine University of Pennsylvania Philadelphia, PA, USA.
Carolann EspyDepartment of Systems Pharmacology and Translational Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Liam S ChaseDivision of Pulmonary, Allergy, and Critical Care, Department of Medicine, Perelman School of Medicine University of Pennsylvania Philadelphia, PA, USA.
Aparajeeta MajumdarDivision of Pulmonary, Allergy, and Critical Care, Department of Medicine, Perelman School of Medicine University of Pennsylvania Philadelphia, PA, USA.
Evan HoffmanDivision of Pulmonary, Allergy, and Critical Care, Department of Medicine, Perelman School of Medicine University of Pennsylvania Philadelphia, PA, USA.
Anit ShahDivision of Pulmonary, Allergy, and Critical Care, Department of Medicine, Perelman School of Medicine University of Pennsylvania Philadelphia, PA, USA.
András SárközyDepartment of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Jeremy KatzenDivision of Pulmonary, Allergy, and Critical Care, Department of Medicine, Perelman School of Medicine University of Pennsylvania Philadelphia, PA, USA.
Norbert PardiDepartment of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Jacob S BrennerDepartment of Systems Pharmacology and Translational Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.ORCID 0000-0001-8437-0161

Funding

NRSA Training CoreTL1TR001880 · NCATS · UNIVERSITY OF PENNSYLVANIA · PI MEAGHER, EMMA ANNE · 2016 to 2025
$10.0M
Lipid nanoparticles as novel adjuvants inducing effective T follicular helper cell and humoral immune responsesR01AI153064 · NIAID · UNIVERSITY OF PENNSYLVANIA · PI Michela Locci, Norbert Pardi · 2020 to 2026
$4.2M
mRNA-LNPs for ARDSR01HL164594 · NHLBI · UNIVERSITY OF PENNSYLVANIA · PI Jacob Brenner, Hamideh Parhiz · 2023 to 2026
$2.7M
Controlling complement to unleash nanomedicine for acute critical illnessesR01HL160694 · NHLBI · UNIVERSITY OF PENNSYLVANIA · PI BRENNER, JACOB, MUZYKANTOV, VLADIMIR R · 2022 to 2025
$2.7M
Nanomedicine for ARDS: A new paradigm to target drugs to multiple cell types within alveolar capillariesR01HL153510 · NHLBI · UNIVERSITY OF PENNSYLVANIA · PI BRENNER, JACOB · 2020 to 2024
$2.0M
NCATS NIH HHS TL1 TR001880NHLBI NIH HHS R01 HL153510NHLBI NIH HHS R01 HL160694NHLBI NIH HHS R01 HL164594NIAID NIH HHS R01 AI153064
6 · The paper itself

Abstract

Lipid nanoparticles (LNPs) have transformed genetic medicine, recently shown by their use in COVID-19 mRNA vaccines. While loading LNPs with mRNA has many uses, loading DNA would provide additional advantages such as long-term expression and availability of promoter sequences. However, here we show that plasmid DNA (pDNA) delivery via LNPs (pDNA-LNPs) induces acute inflammation in naïve mice which we find is primarily driven by the cGAS-STING pathway. Inspired by DNA viruses that inhibit this pathway for replication, we co-loaded endogenous lipids that inhibit STING into pDNA-LNPs. Specifically, loading nitro-oleic acid (NOA) into pDNA-LNPs (NOA-pDNA-LNPs) ameliorates serious inflammatory responses

Identifiers

PMID38915627
PMCPMC11195186

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.