Evidence map›Paper›PMID 38915503›Full record

ArticlebioRxiv : the preprint server for biology2024

Targeted mutagenesis of specific genomic DNA sequences in animals for the

Julia Falo-Sanjuan, Yuliana Diaz-Tirado, Meghan A Turner, Olivia Rourke, Julian Davis, Claudia Medrano, Jenna Haines, Joey McKenna, Arman Karshenas, Michael B Eisen and 1 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Julia Falo-SanjuanDepartment of Molecular and Cell Biology, University of California, Berkeley, CA, USA.ORCID 0000-0002-3563-4789
Yuliana Diaz-TiradoDepartment of Molecular and Cell Biology, University of California, Berkeley, CA, USA.
Meghan A TurnerBiophysics Graduate Group, University of California, Berkeley, CA, USA.ORCID 0000-0003-2451-5036
Olivia RourkeDepartment of Molecular and Cell Biology, University of California, Berkeley, CA, USA.
Julian DavisDepartment of Molecular and Cell Biology, University of California, Berkeley, CA, USA.
Claudia MedranoDepartment of Molecular and Cell Biology, University of California, Berkeley, CA, USA.
Jenna HainesDepartment of Molecular and Cell Biology, University of California, Berkeley, CA, USA.ORCID 0000-0002-4468-3857
Joey McKennaDepartment of Molecular and Cell Biology, University of California, Berkeley, CA, USA.
Arman KarshenasBiophysics Graduate Group, University of California, Berkeley, CA, USA.ORCID 0000-0001-5477-1861
Michael B EisenDepartment of Molecular and Cell Biology, University of California, Berkeley, CA, USA.ORCID 0000-0002-7528-738X
Hernan G GarciaDepartment of Molecular and Cell Biology, University of California, Berkeley, CA, USA.ORCID 0000-0002-5212-3649

Funding

PREP @ UC BerkeleyR25GM140276 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI GIAN GARRIGA, Julia Schaletzky · 2022 to 2026
$1.9M
Predictive understanding of the temporal control of transcription in Drosophila developmentR01GM139913 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI GARCIA, HERNAN GUSTAVO · 2021 to 2024
$1.2M
NIGMS NIH HHS R01 GM139913NIGMS NIH HHS R25 GM140276
6 · The paper itself

Abstract

Understanding how the number, placement and affinity of transcription factor binding sites dictates gene regulatory programs remains a major unsolved challenge in biology, particularly in the context of multicellular organisms. To uncover these rules, it is first necessary to find the binding sites within a regulatory region with high precision, and then to systematically modulate this binding site arrangement while simultaneously measuring the effect of this modulation on output gene expression. Massively parallel reporter assays (MPRAs), where the gene expression stemming from 10,000s of in vitro-generated regulatory sequences is measured, have made this feat possible in high-throughput in single cells in culture. However, because of lack of technologies to incorporate DNA libraries, MPRAs are limited in whole organisms. To enable MPRAs in multicellular organisms, we generated tools to create a high degree of mutagenesis in specific genomic loci

Identifiers

PMID38915503
PMCPMC11195090

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.