ArticleScientific reports2024
RNAi-mediated downregulation of AcCENH3 can induce in vivo haploids in onion (Allium cepa L.).
Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Inducing haploids in rice through OsCENH3 modulation.Science China. Life sciences · 2026Article
- Targeted CENH3 protein depletion in egg cells enables highly efficient haploid induction.Plant communications · 2026Article
- Exploring potential strategies for haploid induction based on double fertilization in plants.Plant biotechnology journal · 2025Review
- Centromeres drive and take a break.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Review
- Direct haploid formation in Arabidopsis using transgenic CENH3-based inducers.Plant cell reports · 2025Article
- Forage Crop Research in the Modern Age.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Haploid Production inBiology · 2025Review
- Past innovations and future possibilities in plant chromosome engineering.Plant biotechnology journal · 2025Review
- Genes and genetics belong to maize haploid induction.Frontiers in plant science · 2025Review
- Pioneers of chromosome elimination.Frontiers in epigenetics and epigenomics · 2025Article
- Recent Progress on Plant Apomixis for Genetic Improvement.International journal of molecular sciences · 2024Review
- Plant kinetochore complex: composition, function, and regulation.Frontiers in plant science · 2024Review
- Haploid induction: an overview of parental factor manipulation during seed formation.Frontiers in plant science · 2024Review
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Authors and funding
9 authors.
Funding
Abstract
Haploid induction (HI) holds great promise in expediting the breeding process in onion, a biennial cross-pollinated crop. We used the CENH3-based genome elimination technique in producing a HI line in onion. Here, we downregulated AcCENH3 using the RNAi approach without complementation in five independent lines. Out of five events, only three could produce seeds upon selfing. The progenies showed poor seed set and segregation distortion, and we were unable to recover homozygous knockdown lines. The knockdown lines showed a decrease in accumulation of AcCENH3 transcript and protein in leaf tissue. The decrease in protein content in transgenic plants was correlated with poor seed set. When the heterozygous knockdown lines were crossed with wild-type plants, progenies showed HI by genome elimination of the parental chromosomes from AcCENH3 knockdown lines. The HI efficiency observed was between 0 and 4.63% in the three events, and it was the highest (4.63%) when E1 line was crossed with wildtype. Given the importance of doubled haploids in breeding programmes, the findings from our study are poised to significantly impact onion breeding.
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