Evidence map›Paper›PMID 38913690›Full record

ArticleThe Journal of infectious diseases2024

Osteopontin Depletion in Nonhematopoietic Cells Improves Outcomes in Septic Mice by Enhancing Antimicrobial Peptide Production.

Chang Yu, Dongwei Xu, Yichun Luo, Junzhe Jiao, Guanjie Liu, Fang Wang, Yueqiu Gao, Xuehua Sun, Xin Lv, Hailong Wu and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of infectious diseases, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Chang YuCentral Laboratory, Department of Liver Diseases, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine.
Dongwei XuDepartment of Liver Surgery, Renji Hospital, Shanghai Jiaotong University School of Medicine.
Yichun LuoCentral Laboratory, Department of Liver Diseases, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine.
Junzhe JiaoCentral Laboratory, Department of Liver Diseases, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine.
Guanjie LiuCentral Laboratory, Department of Liver Diseases, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine.
Fang WangCentral Laboratory, Department of Liver Diseases, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine.
Yueqiu GaoCentral Laboratory, Department of Liver Diseases, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine.
Xuehua SunCentral Laboratory, Department of Liver Diseases, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine.
Xin LvDepartment of Anesthesiology, Shanghai Pulmonary Hospital, Tongji University School of Medicine.
Hailong WuShanghai Key Laboratory for Molecular Imaging, Collaborative Research Center, Shanghai University of Medicine and Health Sciences, China.
Xiaoni KongCentral Laboratory, Department of Liver Diseases, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine.

Funding

National Key Sci-Tech Special Project of China 2018ZX10725-504National Natural Science Foundation of China 82070633Program of Shanghai Academic/Technology Research Leader 20XD1403700
6 · The paper itself

Abstract

Osteopontin (Opn) depletion can improve septic outcomes, but the underlying mechanism remains unknown. In this study, we demonstrated that nonhematopoietic but not hematopoietic Opn depletion improved septic outcomes. When compared with wild type mice, cohoused Opn-/- mice displayed enhanced production of antibacterial peptides (AMPs), decreased bacterial loads, and a distinct bacterial composition of gut microbiota. Fecal microbiota transplantation and OPN neutralization assay showed that Opn depletion could reduce bacterial loads and improve septic inflammation. By employing an intestinal organoid culture system, we proved that OPN neutralization in wild type organoids could inactivate AKT and decrease FOXO3a phosphorylation, resulting in enhanced AMP production, whereas OPN treatment in OPN-deficient organoids could activate AKT and increase FOXO3a phosphorylation, leading to reduced AMP production. Our findings identified OPN as a novel regulatory factor of AMP production to modulate bacterial loads and composition of gut microbiota, in turn affecting sepsis outcomes.

Indexed as

Antimicrobial PeptidesGastrointestinal MicrobiomeOsteopontinSepsisAnimalsBacterial LoadDisease Models, AnimalFecal Microbiota TransplantationForkhead Box Protein O3MaleMiceMice, Inbred C57BLMice, KnockoutOrganoidsPhosphorylationProto-Oncogene Proteins c-aktAntimicrobial PeptidesForkhead Box Protein O3FoxO3 protein, mouseOsteopontinProto-Oncogene Proteins c-aktSpp1 protein, mouseAKT-FOXO signalingantimicrobial peptidesgut microbiotaosteopontinsepsis

Identifiers

PMID38913690
PMCPMC11566238

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.