Studying the Dynamics of Tunneling Tubes and Cellular Spheres.

Sanjana Thanedar, Eric Heng, Donghong Ju, Kezhong Zhang, Henry H Heng

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Sanjana ThanedarCenter for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, MI, USA.

Eric HengStanford University, Stanford, CA, USA.

Donghong JuCenter for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, MI, USA.

Kezhong ZhangCenter for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, MI, USA.

Henry H HengCenter for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, MI, USA. hheng@med.wayne.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cancer cytogenetic analyses often involve cell culture. However, many cytogeneticists overlook interesting phenotypes associated with cultured cells. Given that cytogeneticists need to focus more on phenotypes to comprehend the genotypes, the biological significance of seemingly trivial cellular variations deserves attention. One example is the formation of cellular tunneling tubes (TTs) in cultured cancer cells, which likely play a role in cell-to-cell communication and material transport. In this chapter, we describe protocols for studying these TTs as well as cellular spheres. In addition to diverse chromosomal variants, these different types of variations should be considered for understanding cancer heterogeneity and dynamics, as they illustrate the importance of various forms of fuzzy inheritance.

Indexed as

Cell CommunicationSpheroids, CellularCell Culture TechniquesCell Line, TumorHumansCell communicationCellular heterogeneityFuzzy inheritanceTube networkTunneling nanotubes or TNTs

Identifiers

PMID38913319

DOI10.3390/genes14020493

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.