ArticleChromosoma1985
Monoclonal antibodies to lampbrush chromosome antigens of Pleurodeles waltlii.
Article in Chromosoma, 1985. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 54 citations in OpenAlex.
- Production and characterization of monoclonal antibodies to Xenopus proteins.Development (Cambridge, England) · 2023Article
- A role for SOX9 in post-transcriptional processes: insights from the amphibian oocyte.Scientific reports · 2018Article
- Localized co-transcriptional recruitment of the multifunctional RNA-binding protein CELF1 by lampbrush chromosome transcription units.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2007Article
- Conspicuous accumulation of transcription elongation repressor hrp130/CA150 on the intron-rich Balbiani ring 3 gene.Chromosoma · 2004Article
- Lampbrush chromosomes and associated bodies: new insights into principles of nuclear structure and function.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2002Review
- Assembly of the nuclear transcription and processing machinery: Cajal bodies (coiled bodies) and transcriptosomes.Molecular biology of the cell · 1999Article
- Assembly of lampbrush chromosomes from sperm chromatin.Molecular biology of the cell · 1998Article
- Identification of two RNA-binding proteins in Balbiani ring premessenger ribonucleoprotein granules and presence of these proteins in specific subsets of heterogeneous nuclear ribonucleoprotein particles.Molecular and cellular biology · 1996Article
- A monoclonal antibody against DNA topoisomerase II labels the axial granules of Pleurodeles lampbrush chromosomes.Chromosoma · 1996Article
- A zinc-binding domain is required for targeting the maternal nuclear protein PwA33 to lampbrush chromosome loops.The Journal of cell biology · 1995Article
- Article
- Asymmetrical DNA and AT/GC base content of differential sector of Pleurodeles waltl sexual bivalent: a quantitative fluorescence imaging analysis in lampbrush chromosomes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 1994Article
- hnRNP G: sequence and characterization of a glycosylated RNA-binding protein.Nucleic acids research · 1993Article
- Identification and characterization of a sphere organelle protein.The Journal of cell biology · 1993Article
- A putative zinc-binding protein on lampbrush chromosome loops.The EMBO journal · 1993Article
- Transcription on lampbrush chromosome loops in the absence of U2 snRNA.Molecular biology of the cell · 1992Article
- Immunolocalization of a nuclear protein bound to the sphere organelle during oogenesis and embryogenesis inPleurodeles waltl.Roux's archives of developmental biology : the official organ of the EDBO · 1991Article
- Small nuclear ribonucleoproteins and heterogeneous nuclear ribonucleoproteins in the amphibian germinal vesicle: loops, spheres, and snurposomes.The Journal of cell biology · 1991Article
- The maternally expressed Drosophila gene encoding the chromatin-binding protein BJ1 is a homolog of the vertebrate gene Regulator of Chromatin Condensation, RCC1.The EMBO journal · 1991Article
- A monoclonal antibody that recognizes a phosphorylated epitope stains lampbrush chromosome loops and small granules in the amphibian germinal vesicle.The Journal of cell biology · 1990Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Germinal vesicles of oocytes from Pleurodeles waltlii were used for immunization of BALB/c mice to obtain hybridomas secreting monoclonal antibodies. The hybridomas were screened for reactivity of their antibodies against lampbrush chromosomes of oocytes, as revealed by indirect immunostaining. Antibodies labelling the lampbrush chromosomes were also tested on histological sections of oocytes, embryos, and larvae of Pleurodeles. Characterization of the antigens was accomplished through immunoblotting of two-dimensional electrophoretic gels of germinal vesicle proteins. The ten monoclonal antibodies giving a positive reaction were classed into five groups. Group 1, exemplified by antibody A33, recognizes all the lampbrush chromosome transcribing sites (loops). Moreover, it differentially labels the cell nuclei during embryonic and larval development. Group 2, antibody B71, also stains all the loops of the lampbrush chromosomes, but does not react with cell nuclei of embryos and larvae. Group 3, antibody A1, labels specific loops, some of which are heterozygous in the strain of P. waltlii used. These heterozygosities have allowed us to localize and to characterize a chromosomal segment on bivalent IV which is heteromorphic in the two partners of the bivalent. We suggest that this heteromorphism represents a morphological distinction between Z and W heterochromosomes. Moreover, this antibody reacts with only one transcription unit along a loop that contains several units. Group 4, antibody B24, stains the only two structures in the lampbrush chromosomes of P. waltlii that do not have a loop organization, the mass "M" and the spheres. Group 5, antibody A35, reacts with the chromomeres. The antigens corresponding to antibodies A33 and B24 have been identified as proteins, which have apparent molecular weights of 80 and 104 kilodaltons, respectively. They correspond to proteins abundant in the germinal vesicles. All the antibodies described here cross-react with the lampbrush chromosomes of five other species of Urodeles.
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