Evidence map›Paper›PMID 38912171›Full record

ArticleBiomedical reports2024

Evaluation of silica spin‑column and magnetic bead formats for rapid DNA methylation analysis in clinical and point‑of‑care settings.

Fernando T Zamuner, Ashley Ramos-López, Amanda García-Negrón, Ana Purcell-Wiltz, Andrea Cortés-Ortiz, Aniris Román Cuevas, Keerthana Gosala, Eli Winkler, David Sidransky, Rafael Guerrero-Preston

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Fernando T ZamunerDepartment of Otolaryngology and Head and Neck Surgery, Head and Neck Cancer Research Laboratory, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
Ashley Ramos-LópezLifeGene-Biomarks, Research and Development Unit, Toa Baja 00949, Puerto Rico.
Amanda García-NegrónLifeGene-Biomarks, Research and Development Unit, Toa Baja 00949, Puerto Rico.
Ana Purcell-WiltzLifeGene-Biomarks, Research and Development Unit, Toa Baja 00949, Puerto Rico.
Andrea Cortés-OrtizLifeGene-Biomarks, Research and Development Unit, Toa Baja 00949, Puerto Rico.
Aniris Román CuevasLifeGene-Biomarks, Research and Development Unit, Toa Baja 00949, Puerto Rico.
Keerthana GosalaDepartment of Otolaryngology and Head and Neck Surgery, Head and Neck Cancer Research Laboratory, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
Eli WinklerDepartment of Otolaryngology and Head and Neck Surgery, Head and Neck Cancer Research Laboratory, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
David SidranskyDepartment of Otolaryngology and Head and Neck Surgery, Head and Neck Cancer Research Laboratory, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
Rafael Guerrero-PrestonLifeGene-Biomarks, Research and Development Unit, Toa Baja 00949, Puerto Rico.

Funding

Precision methylation biomarkers for cervical cancer prevention in low resource settings in Latin AmericaR44CA254690 · NCI · LIFEGENE-BIOMARKS, INC. · PI GUERRERO-PRESTON, RAFAEL · 2021 to 2023
$2.4M
Precision screening in self-collected samples to reduce cervical cancer disparities among LatinasR42MD018231 · NIMHD · LIFEGENE-BIOMARKS, INC. · PI Rafael Guerrero-Preston · 2024 to 2026
$2.1M
Precision methylation biomarkers linked to cancer disparitiesR44MD014911 · NIMHD · LIFEGENE-BIOMARKS, INC. · PI GUERRERO-PRESTON, RAFAEL · 2019 to 2021
$1.7M
NCI NIH HHS R44 CA254690NIMHD NIH HHS R42 MD018231NIMHD NIH HHS R44 MD014911
6 · The paper itself

Abstract

Late-stage cancers lack effective treatment, underscoring the need for early diagnosis to improve prognosis and decrease mortality rates. Molecular markers, such as DNA methylation, offer promise in early cancer detection. The present study compared commercial kits for analyzing DNA from cervical liquid cytology samples in cancer screening. Rapid bisulfite conversion kits using silica spin-columns and magnetic beads were assessed against standard DNA extraction and bisulfite conversion methods for profiling DNA methylation using quantitative methylation-specific PCR.

Indexed as

cervical cancerDNA methylationhuman papillomavirusliquid cytologymethylation kitmolecular markerprecision medicinepremalignant lesionquantitative methylation-specific PCRscreening method

Identifiers

PMID38912171
PMCPMC11190640

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.