Evidence map›Paper›PMID 38909053›Full record

ArticleNature communications2024

Strain dynamics of contaminating bacteria modulate the yield of ethanol biorefineries.

Felipe Senne de Oliveira Lino, Shilpa Garg, Simone S Li, Maria-Anna Misiakou, Kang Kang, Bruno Labate Vale da Costa, Tobias Svend-Aage Beyer-Pedersen, Thamiris Guerra Giacon, Thiago Olitta Basso, Gianni Panagiotou and 1 more

Abstract read
In one paragraph

Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Microbiology resource announcements · 2026
    Article
  3. Dynamics of contaminant microbes in bioethanol production from sugarcane.Journal of industrial microbiology & biotechnology · 2026
    Article
  4. Review
  5. Endogenous Ethanol Metabolism and Development of MASLD-MASH.International journal of molecular sciences · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Felipe Senne de Oliveira Lino *The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, 2800, Denmark.ORCID 0000-0001-5645-9142
Shilpa Garg *The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, 2800, Denmark.ORCID 0000-0003-0200-4200
Simone S LiThe Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, 2800, Denmark.
Maria-Anna MisiakouThe Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, 2800, Denmark.
Kang KangLeibniz Institute for Natural Product Research and Infection Biology, Jena, 07745, Germany.ORCID 0000-0002-7992-282X
Bruno Labate Vale da CostaEscola de Engenharia de Alimentos da Universidade de Campinas, 13083-862, Campinas, SP, Brazil.
Tobias Svend-Aage Beyer-PedersenThe Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, 2800, Denmark.ORCID 0009-0002-2769-5570
Thamiris Guerra GiaconDepartamento de Engenharia Química da Escola Politécnica da Universidade de São Paulo. Universidade de São Paulo, 05508-000, São Paulo, SP, Brazil.ORCID 0000-0002-2025-3764
Thiago Olitta BassoDepartamento de Engenharia Química da Escola Politécnica da Universidade de São Paulo. Universidade de São Paulo, 05508-000, São Paulo, SP, Brazil.ORCID 0000-0001-7765-4297
Gianni PanagiotouLeibniz Institute for Natural Product Research and Infection Biology, Jena, 07745, Germany.ORCID 0000-0001-9393-124X
Morten Otto Alexander SommerThe Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, 2800, Denmark. msom@bio.dtu.dk.ORCID 0000-0003-4005-5674

Funding

Department of Health | National Health and Medical Research Council (NHMRC) APP1166180European Molecular Biology Organization (EMBO) ALTF 137-2018Ministry of Science, Technology and Innovation | Conselho Nacional de Desenvolvimento Científico e Tecnológico (National Council for Scientific and Technological Development) 406564/2022-1Novo Nordisk Fonden (Novo Nordisk Foundation) NNF10CC1016517
6 · The paper itself

Abstract

Bioethanol is a sustainable energy alternative and can contribute to global greenhouse-gas emission reductions by over 60%. Its industrial production faces various bottlenecks, including sub-optimal efficiency resulting from bacteria. Broad-spectrum removal of these contaminants results in negligible gains, suggesting that the process is shaped by ecological interactions within the microbial community. Here, we survey the microbiome across all process steps at two biorefineries, over three timepoints in a production season. Leveraging shotgun metagenomics and cultivation-based approaches, we identify beneficial bacteria and find improved outcome when yeast-to-bacteria ratios increase during fermentation. We provide a microbial gene catalogue which reveals bacteria-specific pathways associated with performance. We also show that Limosilactobacillus fermentum overgrowth lowers production, with one strain reducing yield by ~5% in laboratory fermentations, potentially due to its metabolite profile. Temperature is found to be a major driver for strain-level dynamics. Improved microbial management strategies could unlock environmental and economic gains in this US $ 60 billion industry enabling its wider adoption.

Indexed as

BacteriaEthanolFermentationBiofuelsIndustrial MicrobiologyMetagenomicsMicrobiotaTemperatureBiofuelsEthanol

Identifiers

PMID38909053
PMCPMC11193817

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.