Evidence map›Paper›PMID 38907823›Full record

ArticleJournal of endocrinological investigation2024

JPT2 in subclinical hypothyroidism-related miscarriage as a transcription co-factor: involvement of LEPR/STAT3 activation.

Y-Y Zhou, S-Y Zhao, F-J Huang, L-J Zhang, Y-L Liu, J Wang, X-J Ma

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Article in Journal of endocrinological investigation, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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0cells of the map it votes in
1citing papers in PubMed
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1 · What the graph read from it

What it found

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Y-Y ZhouDepartment of Endocrinology and Metabolism, the First Affiliated Hospital of Zhengzhou University, No. 1, East Jianshe Road, Zhengzhou, 450000, Henan Province, People's Republic of China.
S-Y ZhaoDepartment of Endocrinology and Metabolism, the First Affiliated Hospital of Zhengzhou University, No. 1, East Jianshe Road, Zhengzhou, 450000, Henan Province, People's Republic of China.
F-J HuangDepartment of Endocrinology and Metabolism, the First Affiliated Hospital of Zhengzhou University, No. 1, East Jianshe Road, Zhengzhou, 450000, Henan Province, People's Republic of China.
L-J ZhangDepartment of Endocrinology and Metabolism, the First Affiliated Hospital of Zhengzhou University, No. 1, East Jianshe Road, Zhengzhou, 450000, Henan Province, People's Republic of China.
Y-L LiuDepartment of Endocrinology and Metabolism, the First Affiliated Hospital of Zhengzhou University, No. 1, East Jianshe Road, Zhengzhou, 450000, Henan Province, People's Republic of China.
J WangDepartment of Endocrinology and Metabolism, the First Affiliated Hospital of Zhengzhou University, No. 1, East Jianshe Road, Zhengzhou, 450000, Henan Province, People's Republic of China.
X-J MaDepartment of Endocrinology and Metabolism, the First Affiliated Hospital of Zhengzhou University, No. 1, East Jianshe Road, Zhengzhou, 450000, Henan Province, People's Republic of China. fccmaxj1@zzu.edu.cn.ORCID http://orcid.org/0000-0001-8348-3842

Funding

Grants from the Science and Technology Project of Henan Province 2018020010Grants from the Science and Technology Project of Henan Province LHGJ20190257National Natural Science Foundation of China 81900715National Natural Science Foundation of China 82270927National Natural Science Foundation of China 82300883
6 · The paper itself

Abstract

background and purposeSubclinical hypothyroidism (SCH) has been identified to be associated with implantation failure, in which the dysfunction of trophoblast cells is involved. In this study, the transcriptomics of aborted placenta from SCH rats were analyzed. Jupiter microtubule-associated homolog 2 (JPT2) was downregulated in the aborted placenta. This study aims to investigate its role in SCH-associated miscarriage.

methodsSpontaneous abortion was observed in SCH rats generated by thyroidectomy combined with levothyroxine administration. The transcriptomics analysis was performed using aborted placenta. Afterward, the effects of JPT2 on trophoblast cells were explored using gain-and loss-of-function experiments.

resultsTranscriptomics analysis showed 1286 downregulated genes and 2300 upregulated genes in the aborted placenta, and JPT2 was significantly downregulated in the aborted placenta from SCH rats. Afterward, gain-and loss-of-function experiments exhibited that overexpression of JPT2 promoted the proliferation, migration, invasion, spheroid formation of HTR-8/SVneo trophoblast cells and their attachment to endometrial stromal cells, while these biological behaviors were suppressed by JPT2 knockdown. Furthermore, JPT2 accelerated the transcription of leptin receptor (LEPR), and activated signal transducer and activator of transcription 3 (STAT3) signal in a transcription factor AP-2γ-dependent manner. In addition, silencing of LEPR abolished the role of JPT2.

conclusionOur results revealed that JPT2, which was downregulated in the aborted placenta from SCH rats, promoted proliferation, migration, invasion, spheroid formation, and attachment of trophoblast cells via regulating LEPR/STAT3 axis as a transcription co-factor. It is indicated that low expression of JPT2 may contribute to the abortion in individuals with SCH.

Indexed as

Abortion, SpontaneousHypothyroidismSTAT3 Transcription FactorAnimalsCell ProliferationFemaleHumansMicrotubule-Associated ProteinsPlacentaPregnancyRatsRats, Sprague-DawleyReceptors, LeptinSignal TransductionTrophoblastsMicrotubule-Associated ProteinsReceptors, LeptinStat3 protein, ratSTAT3 Transcription FactorInvasionJupiter microtubule-associated homolog 2Leptin receptorSTAT3Trophoblast cell

Identifiers

PMID38907823

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.